10.1371/journal.pone.0158196.g005
Sonia Nasi
Sonia
Nasi
Hang-Korng Ea
Hang-Korng
Ea
Frédéric Lioté
Frédéric
Lioté
Alexander So
Alexander
So
Nathalie Busso
Nathalie
Busso
STS inhibits formation of new calcific deposits and cartilage degradation in experimental OA.
Public Library of Science
2016
RT-PCR
Annexin V gene expression
OA
dose-dependent manner calcium crystal deposition
cartilage damage
crystals-stimulated murine chondrocytes
HA-induced ROS generation
reactive oxygen species
crystal-induced IL -6
HA-induced catabolic responses
cartilage matrix degradation
periarticular calcific deposits
STS
calcification
Murine Osteoarthritis Objectives Calcium-containing crystals
MCP
2016-07-08 16:38:16
Figure
https://plos.figshare.com/articles/figure/STS_inhibits_formation_of_new_calcific_deposits_and_cartilage_degradation_in_experimental_OA_/3906102
<p>(<b>a</b>) Representative micro-CT scan images of menisectomized murine knee joints after vehicle (PBS) or STS treatment for two months after surgery. White arrows show periarticular deposits in menisectomized knees treated with vehicle and their reduction in STS treated mice. Graphs show CTAnalyzer quantitative analysis of new formation volume (mm<sup>3</sup>) and new formation crystal content (μg) in PBS- and STS-treated menisectomized mice. Data are expressed as the mean±SD (<b>b</b>) Representative histologies of PBS- and STS-treated menisectomized knees, stained with Safranin-O. Red arrows show degenerative OA changes in the articular cartilage of PBS-treated mice. Scale bars 150 μm. Graphs show tibial and femoral scoring of cartilage damage and Safranin-O loss, accordingly to OARSI method. Values represent means±SD. (<b>c</b>) Correlation graph between tibial cartilage damage score and new formation volume of pooled PBS- and STS-treated mice. (Mice number: PBS n = 20, STS n = 19).</p>