10.1371/journal.pone.0160074.g006 Valentina Masola Valentina Masola Gianluigi Zaza Gianluigi Zaza Giovanni Gambaro Giovanni Gambaro Maurizio Onisto Maurizio Onisto Gloria Bellin Gloria Bellin Gisella Vischini Gisella Vischini Iyad Khamaysi Iyad Khamaysi Ahmad Hassan Ahmad Hassan Shadi Hamoud Shadi Hamoud Omri Nativ Omri Nativ Samuel N. Heyman Samuel N. Heyman Antonio Lupo Antonio Lupo Israel Vlodavsky Israel Vlodavsky Zaid Abassi Zaid Abassi <i>In vivo</i> Heparanase (HPSE) expression induced by Ischemia and Reperfusion (I/R) kidney injury. Public Library of Science 2016 FGF -2 graft function histological methodologies mesenchymal transition VIM 30 min WT littermates vivo models TGF β-induced EMT FN WT animals HPA-tg mice 72 h Potential New Factor SMA EMT markers pro-fibrotic machinery Renal Epithelial Mesenchymal Transition Methods Wild type mice over-expressing HPSE SST 0001 treatment WT cells disease progression 2016-07-28 12:26:44 Figure https://plos.figshare.com/articles/figure/_i_In_vivo_i_Heparanase_HPSE_expression_induced_by_Ischemia_and_Reperfusion_I_R_kidney_injury_/3877980 <p><b>A)</b> Box plot representing relative gene expression of HPSE evaluated by real-time PCR in renal tissue extract from Wild type (WT) mice. Results were normalized to GAPDH expression. Mean ± S.E (error bars) of two separate experiments performed in triplicate. <b>B)</b> Representative immunofluorescence staining for HPSE in cortical renal tissues of WT mice 72 h after sham operation or I/R kidney injury. I/R, ischemia/reperfusion. <b>C)</b> Quantification of the immunofluorescence staining. **p<0.001vs. WT SHAM.</p>