Flow diagram of the main experimental design. YousefMohamed Samy MareyMohamed Ali HambruchNina HayakawaHiroyuki ShimizuTakashi HussienHassan Ali Abdel-RazekAbdel-Razek Khalifa PfarrerChristiane MiyamotoAkio 2016 <p>BOEC (bovine oviduct epithelial cells) monolayers co-cultured with sperm at different time points (3, 6, 12 and 24 h)<b>.</b> PMNs (polymorphonuclear neutrophils) isolated from whole blood and co-cultured for 4 h with BOEC-conditioned media. The cells were collected to analyse immune-related gene expressions using real-time PCR.</p>