Supplementary Material for: Phosphorylation of STAT3 in Undifferentiated Pleomorphic Sarcoma Is Correlated with a Favorable Prognosis Bekki H. Kohashi K. Yamada Y. Iura K. Ishii T. Maekawa A. Otsuka H. Yamamoto H. Hakozaki M. Nabeshima K. Iwamoto Y. Oda Y. 10.6084/m9.figshare.3843489.v1 https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Phosphorylation_of_STAT3_in_Undifferentiated_Pleomorphic_Sarcoma_Is_Correlated_with_a_Favorable_Prognosis/3843489 <p><strong><em>Objective:</em></strong> The Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway plays a role in various biological processes. Phosphorylated STAT3 (p-STAT3) functions as a transcriptional factor, and suppressor of cytokine signaling 3 (SOCS3) is a potential inhibitor of STAT3. Here, we analyzed the status of the JAK-STAT pathway in undifferentiated pleomorphic sarcoma (UPS). <b><i>Methods:</i></b> We performed immunohistochemistry in 79 samples of UPS and Western blotting in 10 frozen samples. We also examined alterations in protein expression in the JAK-STAT pathway after the inhibition of phosphorylated Akt (p-Akt) or extracellular signal-regulated kinase (p-Erk) in vitro. <b><i>Results:</i></b> Immunohistochemically, p-STAT3 and SOCS3 were positive in 59.7 and 55.8%, respectively. Positivity for p-STAT3 was significantly correlated with a better prognosis (p = 0.0006) and negatively with SOCS3 expression (p = 0.0223). Positivity for SOCS3 was significantly correlated with a worse prognosis (p = 0.0001). Western blotting analysis revealed that p-STAT3 expression was lower in tumor than in normal tissue. In vitro results demonstrated that there was no detectable change in the expression of p-STAT3 regardless of the status of p-Akt or p-Erk. <b><i>Conclusion:</i></b> p-STAT3 may be a useful prognostic factor for UPS.</p> 2016-09-21 12:38:33 JAK-STAT pathway SOCS3 STAT3 Undifferentiated pleomorphic sarcoma