MrkH and MrkI are required for repression of CRP in type 3 fimbriae.
Ching-Ting Lin
Tien-Huang Lin
Chien-Chen Wu
Lei Wan
Chun-Fa Huang
Hwei-Ling Peng
10.1371/journal.pone.0162884.g004
https://plos.figshare.com/articles/figure/MrkH_and_MrkI_are_required_for_repression_of_CRP_in_type_3_fimbriae_/3835419
<p>(A) qRT-PCR analysis of <i>mrkA</i> expression was measured in WT, Δ<i>crp</i>, Δ<i>crp</i>Δ<i>mrkH</i>, Δ<i>crp</i>Δ<i>mrkI</i>, and Δ<i>crp</i>Δ<i>mrkJ</i> strains in LB medium. The results are representative of three independent experiments. Error bars indicate standard deviations. *<i>P</i> < 0.05 and ** <i>P</i> < 0.01 compared to the indicated group. The MrkA expression of WT, Δ<i>crp</i>, Δ<i>crp</i>Δ<i>mrkH</i>, Δ<i>crp</i>Δ<i>mrkI</i>, and Δ<i>crp</i>Δ<i>mrkJ</i> strains was determined in LB broth without (B) or with 0.5% glucose (C) by Western blot analysis against MrkA (the upper panel) and GAPDH antiserum (the lower panel, for internal control). The MrkA and GAPDH proteins are indicated by an arrow, respectively.</p>
2016-09-15 17:39:43
mrkABCDF genes
influence type 3 fimbriae expression
CRP-cAMP down-regulates mrkABCDF
Δ crp strain
type 3 fimbriae expression regulation
type 3 fimbriae
encode cyclic di-GMP
type 3 fimbriae expression
P mrkHI
mRNA levels
Type 3 fimbriae
blood glucose levels
Type 3 Fimbriae
Δ lacZ Δ crp Δ mrkHI
Klebsiella pneumoniae Klebsiella pneumoniae
CRP-Cyclic AMP Regulates
intracellular cyclic AMP
intracellular c-di-GMP concentration
Δ lacZ Δ crp strain
Δ lacZ Δ crp Δ mrkJ strain