MrkH and MrkI are required for repression of CRP in type 3 fimbriae. Ching-Ting Lin Tien-Huang Lin Chien-Chen Wu Lei Wan Chun-Fa Huang Hwei-Ling Peng 10.1371/journal.pone.0162884.g004 https://plos.figshare.com/articles/figure/MrkH_and_MrkI_are_required_for_repression_of_CRP_in_type_3_fimbriae_/3835419 <p>(A) qRT-PCR analysis of <i>mrkA</i> expression was measured in WT, Δ<i>crp</i>, Δ<i>crp</i>Δ<i>mrkH</i>, Δ<i>crp</i>Δ<i>mrkI</i>, and Δ<i>crp</i>Δ<i>mrkJ</i> strains in LB medium. The results are representative of three independent experiments. Error bars indicate standard deviations. *<i>P</i> < 0.05 and ** <i>P</i> < 0.01 compared to the indicated group. The MrkA expression of WT, Δ<i>crp</i>, Δ<i>crp</i>Δ<i>mrkH</i>, Δ<i>crp</i>Δ<i>mrkI</i>, and Δ<i>crp</i>Δ<i>mrkJ</i> strains was determined in LB broth without (B) or with 0.5% glucose (C) by Western blot analysis against MrkA (the upper panel) and GAPDH antiserum (the lower panel, for internal control). The MrkA and GAPDH proteins are indicated by an arrow, respectively.</p> 2016-09-15 17:39:43 mrkABCDF genes influence type 3 fimbriae expression CRP-cAMP down-regulates mrkABCDF Δ crp strain type 3 fimbriae expression regulation type 3 fimbriae encode cyclic di-GMP type 3 fimbriae expression P mrkHI mRNA levels Type 3 fimbriae blood glucose levels Type 3 Fimbriae Δ lacZ Δ crp Δ mrkHI Klebsiella pneumoniae Klebsiella pneumoniae CRP-Cyclic AMP Regulates intracellular cyclic AMP intracellular c-di-GMP concentration Δ lacZ Δ crp strain Δ lacZ Δ crp Δ mrkJ strain