ESM Supplementary Legends Figures and Table from A model for the effects of germanium on silica biomineralization in choanoflagellates Alan O. Marron Helen Chappell Sarah Ratcliffe Raymond E. Goldstein 10.6084/m9.figshare.3823680.v1 https://rs.figshare.com/articles/journal_contribution/ESM_Supplementary_Legends_Figures_and_Table_from_A_model_for_the_effects_of_germanium_on_silica_biomineralization_in_choanoflagellates/3823680 Silica biomineralization is a widespread phenomenon of major biotechnological interest. Modifying biosilica with substances like germanium (Ge) can confer useful new properties, although exposure to high levels of Ge disrupts normal biosilicification. No clear mechanism explains why this disruption occurs. Here we study the effect of Ge on loricate choanoflagellates, a group of protists that construct a species-specific extracellular lorica from multiple siliceous costal strips. High Ge exposures were toxic, whereas lower Ge exposures produced cells with incomplete or absent loricae. These effects can be ameliorated by restoring the Germanium : Silicon ratio, as observed in other biosilicifying organisms. We developed simulations of how Ge interacts with polymerizing silica. In our models, Ge is readily incorporated at the ends of silica forming from silicic acid condensation, but this prevents further silica polymerization. Our ‘Ge-capping’ model is supported by observations from loricate choanoflagellates. Ge exposure terminates costal strip synthesis and lorica formation, resulting in disruption to cytokinesis and fatal build-up of silicic acid. Applying the Ge-capping model to other siliceous organisms explains the general toxicity of Ge and identifies potential protective responses in metalloid uptake and sensing. This can improve the design of new silica biomaterials, and further our understanding of silicon metabolism. 2016-09-13 07:41:36 germanium silicon biomineralization toxicity first principles modelling choanoflagellate