Ishii, Tasuku Funato, Yosuke Hashizume, Osamu Yamazaki, Daisuke Hirata, Yusuke Nishiwaki, Kiyoji Kono, Nozomu Arai, Hiroyuki Miki, Hiroaki Localization of CNNM-1 and CNNM-3 at the basolateral membrane of intestinal cells. <p>(A) Fluorescent images of wild-type L1 larvae with <i>cnnm-1p</i>::<i>GFP</i> or <i>cnnm-3p</i>::<i>GFP</i>. Areas of neurons and intestines showing strong GFP signals are marked. Bar, 30 μm. (B) Quantification of sterile phenotype in wild-type, <i>cnnm-1</i>; <i>cnnm-3</i> mutants, and <i>cnnm-1</i>; <i>cnnm-3</i> mutants expressing either CNNM-1 or CNNM-3 under the control of the promoters for intestinal (<i>ges-1p</i>) or pan-neuronal (<i>aex-3p</i>) markers. More than 50 worms were analyzed for each genotype. (C) Fluorescent images of intestinal cells from wild-type L1 larvae expressing the Venus-fusion proteins of CNNM-1 or CNNM-3 (left). Schematic representation of the intestinal cell plasma membranes are also shown (right). Apical and basolateral membranes are indicated by dotted and solid lines, respectively. Bar, 10 μm.</p> CNNM;Mg;genes encoding cyclin M;AMPK;Caenorhabditis elegans strains;Caenorhabditis elegans cnnm family genes;gonadogenesis defect 2016-08-26
    https://plos.figshare.com/articles/figure/Localization_of_CNNM-1_and_CNNM-3_at_the_basolateral_membrane_of_intestinal_cells_/3790944
10.1371/journal.pgen.1006276.g005