10.1371/journal.pgen.1006276.g005 Tasuku Ishii Tasuku Ishii Yosuke Funato Yosuke Funato Osamu Hashizume Osamu Hashizume Daisuke Yamazaki Daisuke Yamazaki Yusuke Hirata Yusuke Hirata Kiyoji Nishiwaki Kiyoji Nishiwaki Nozomu Kono Nozomu Kono Hiroyuki Arai Hiroyuki Arai Hiroaki Miki Hiroaki Miki Localization of CNNM-1 and CNNM-3 at the basolateral membrane of intestinal cells. Public Library of Science 2016 CNNM Mg genes encoding cyclin M AMPK Caenorhabditis elegans strains Caenorhabditis elegans cnnm family genes gonadogenesis defect 2016-08-26 17:30:01 Figure https://plos.figshare.com/articles/figure/Localization_of_CNNM-1_and_CNNM-3_at_the_basolateral_membrane_of_intestinal_cells_/3790944 <p>(A) Fluorescent images of wild-type L1 larvae with <i>cnnm-1p</i>::<i>GFP</i> or <i>cnnm-3p</i>::<i>GFP</i>. Areas of neurons and intestines showing strong GFP signals are marked. Bar, 30 μm. (B) Quantification of sterile phenotype in wild-type, <i>cnnm-1</i>; <i>cnnm-3</i> mutants, and <i>cnnm-1</i>; <i>cnnm-3</i> mutants expressing either CNNM-1 or CNNM-3 under the control of the promoters for intestinal (<i>ges-1p</i>) or pan-neuronal (<i>aex-3p</i>) markers. More than 50 worms were analyzed for each genotype. (C) Fluorescent images of intestinal cells from wild-type L1 larvae expressing the Venus-fusion proteins of CNNM-1 or CNNM-3 (left). Schematic representation of the intestinal cell plasma membranes are also shown (right). Apical and basolateral membranes are indicated by dotted and solid lines, respectively. Bar, 10 μm.</p>