10.1371/journal.pgen.1006276.g005
Tasuku Ishii
Tasuku
Ishii
Yosuke Funato
Yosuke
Funato
Osamu Hashizume
Osamu
Hashizume
Daisuke Yamazaki
Daisuke
Yamazaki
Yusuke Hirata
Yusuke
Hirata
Kiyoji Nishiwaki
Kiyoji
Nishiwaki
Nozomu Kono
Nozomu
Kono
Hiroyuki Arai
Hiroyuki
Arai
Hiroaki Miki
Hiroaki
Miki
Localization of CNNM-1 and CNNM-3 at the basolateral membrane of intestinal cells.
Public Library of Science
2016
CNNM
Mg
genes encoding cyclin M
AMPK
Caenorhabditis elegans strains
Caenorhabditis elegans cnnm family genes
gonadogenesis defect
2016-08-26 17:30:01
Figure
https://plos.figshare.com/articles/figure/Localization_of_CNNM-1_and_CNNM-3_at_the_basolateral_membrane_of_intestinal_cells_/3790944
<p>(A) Fluorescent images of wild-type L1 larvae with <i>cnnm-1p</i>::<i>GFP</i> or <i>cnnm-3p</i>::<i>GFP</i>. Areas of neurons and intestines showing strong GFP signals are marked. Bar, 30 μm. (B) Quantification of sterile phenotype in wild-type, <i>cnnm-1</i>; <i>cnnm-3</i> mutants, and <i>cnnm-1</i>; <i>cnnm-3</i> mutants expressing either CNNM-1 or CNNM-3 under the control of the promoters for intestinal (<i>ges-1p</i>) or pan-neuronal (<i>aex-3p</i>) markers. More than 50 worms were analyzed for each genotype. (C) Fluorescent images of intestinal cells from wild-type L1 larvae expressing the Venus-fusion proteins of CNNM-1 or CNNM-3 (left). Schematic representation of the intestinal cell plasma membranes are also shown (right). Apical and basolateral membranes are indicated by dotted and solid lines, respectively. Bar, 10 μm.</p>