10.1371/journal.pone.0028499.g004
Pasqua Gramegna
Pasqua
Gramegna
Tiziana Latronico
Tiziana
Latronico
Maria Teresa BranĂ
Maria
Teresa BranĂ
Gaetano Di Bari
Gaetano
Di Bari
Fabio Mengoni
Fabio
Mengoni
Valeria Belvisi
Valeria
Belvisi
Maria T. Mascellino
Maria
T. Mascellino
Miriam Lichtner
Miriam
Lichtner
Vincenzo Vullo
Vincenzo
Vullo
Claudio M. Mastroianni
Claudio
M. Mastroianni
Grazia M. Liuzzi
Grazia
M. Liuzzi
Zymographic analysis of MMP-2 and MMP-9 activity after <i>in vitro</i> incubation with MVC.
Public Library of Science
2013
mmp-2
mmp-9
incubation
2013-02-20 11:03:17
Figure
https://plos.figshare.com/articles/figure/_Zymographic_analysis_of_MMP_2_and_MMP_9_activity_after_in_vitro_incubation_with_MVC_/376002
<p>Purified standard MMP-2 (0.35 ng) and MMP-9 (0.88 ng) were subjected to gelatin zymography as described in the text. After the electrophoresis, the gel was cut into lanes and each lane was incubated in the absence (control) or in the presence with MVC at the reported concentrations during the development of the zymograms. 1,10 phenantroline (4 mM) (PA) was used as a positive control. A: Staining and destaining of the gels revealed that inhibition of both MMP-2 and MMP-9 activity was observed only in the lane incubated in the presence of PA, but not in the lanes developed in the presence of MVC. B: Percentage of MMP-9 and MMP-2 inhibition of repeated analyses calculated in comparison to control.</p>