%0 Figure %A Pei, Yonggang %A Banerjee, Shuvomoy %A Sun, Zhiguo %A Chandra Jha, Hem %A Saha, Abhik %A S. Robertson, Erle %D 2016 %T E2F6 binds to both amino and carboxy terminal domains of EBNA3C. %U https://plos.figshare.com/articles/figure/E2F6_binds_to_both_amino_and_carboxy_terminal_domains_of_EBNA3C_/3750153 %R 10.1371/journal.ppat.1005844.g003 %2 https://ndownloader.figshare.com/files/5844633 %K E 2F transcription factors %K EBNA 3C cell proliferation %K E 2F promoter %K E 2F expression %K antigen 3 C %K EBNA 3C %K E 2F binding %K LCL %K Inhibit E 2F Transcription %K E 2F genes %K DNA %K carboxy terminal domains %K E 2F forms %K E 2F transcriptional activity %K E 2F transcription regulator %K E 2F family members %K E 2F protein %K E 2F activities %K EBV Nuclear Antigen 3 C Mediates Regulation %K E 2F %X

A) Myc-tagged EBNA3C full length and different truncated mutants were translated in vitro using a T7-TNT kit. The S35-radiolabeled in vitro-translated proteins were pre-cleared with GST-beads, then incubated with GST control or GST-E2F6 protein and GST beads. The mixture were resolved by 10% SDS-PAGE, exposed to phosphorImager plates and scanned with Typhoon Scanner. B-C) Cell lysates form 50 million different B-cells (BJAB, BJAB7, BJAB10, LCL1, LCL2) were collected and incubated with either GST control or GST-E2F6 protein and GST beads. The mixture samples were identified by western blot analysis using specific mouse antibody for EBNA3C (A10). D) Purified GST control and GST-E2F6 proteins were separated by 10% SDS-PAGE and stained with Coomassie Blue. E) The schematic diagram shows various interactive domains of EBNA3C with E2F6.

%I PLOS Pathogens