TY - DATA T1 - Recognition of the Nonpolar Base 4-Methylindole in DNA by the DNA Repair Adenine Glycosylase MutY PY - 2000/04/14 AU - Cindy Lou Chepanoske AU - Charles R. Langelier AU - Nikolas H. Chmiel AU - Sheila S. David UR - https://acs.figshare.com/articles/journal_contribution/Recognition_of_the_Nonpolar_Base_4-Methylindole_in_DNA_by_the_DNA_Repair_Adenine_Glycosylase_MutY/3736077 DO - 10.1021/ol005831o.s001 L4 - https://ndownloader.figshare.com/files/5827845 KW - DNA Repair Adenine Glycosylase MutY KW - MutY exhibit KW - DNA repair adenine glycosylase MutY KW - substrate recognition process KW - OG N2 - The DNA repair adenine glycosylase MutY efficiently recognizes 7-deaza-2‘-deoxyadenosine (Z) and its nonpolar isostere 4-methylindole β-deoxynucleoside (M) opposite 7,8-dihydro-8-oxo-2‘-deoxyguanosine (OG) and G in DNA. Both wild-type and truncated MutY exhibit a 10- to 20-fold higher affinity for a duplex containing OG:M than OG:Z. More efficient recognition of M over Z by MutY may be to due the lack of hydrogen bonding with the OG that facilitates nucleotide flipping during the substrate recognition process. ER -