TY - DATA T1 - Gold Nanoparticle-Based Quantitative Electrochemical Detection of Amplified Human Cytomegalovirus DNA Using Disposable Microband Electrodes PY - 2001/08/10 AU - Laurent Authier AU - Céline Grossiord AU - Pierre Brossier AU - Benoît Limoges UR - https://acs.figshare.com/articles/journal_contribution/Gold_Nanoparticle-Based_Quantitative_Electrochemical_Detection_of_Amplified_Human_Cytomegalovirus_DNA_Using_Disposable_Microband_Electrodes/3582690 DO - 10.1021/ac0103221.s001 L4 - https://ndownloader.figshare.com/files/5668569 KW - nanoparticle probe KW - Amplified Human Cytomegalovirus DNA KW - nonlinear diffusion KW - III mass transfer KW - gold nanoparticle probe KW - 5 pM KW - cytomegalovirus DNA sequence KW - SPMBE KW - oxidative metal dissolution KW - Disposable Microband Electrodes KW - III ions KW - HCMV DNA KW - electrochemical DNA detection method KW - electrodeposition time KW - HCMV DNA fragment KW - III determination KW - gold metal atoms N2 - An electrochemical DNA detection method has been developed for the sensitive quantification of an amplified 406-base pair human cytomegalovirus DNA sequence (HCMV DNA). The assay relies on (i) the hybridization of the single-stranded target HCMV DNA with an oligonucleotide-modified Au nanoparticle probe, (ii) followed by the release of the gold metal atoms anchored on the hybrids by oxidative metal dissolution, and (iii) the indirect determination of the solubilized AuIII ions by anodic stripping voltammetry at a sandwich-type screen-printed microband electrode (SPMBE). Due to the enhancement of the AuIII mass transfer by nonlinear diffusion during the electrodeposition time, the SPMBE allows the sensitive determination of AuIII in a small volume of quiescent solution. The combination of the sensitive AuIII determination at a SPMBE with the large number of AuIII released from each gold nanoparticle probe allows detection of as low as 5 pM amplified HCMV DNA fragment. ER -