%0 Journal Article
%A Authier, Laurent
%A Grossiord, Céline
%A Brossier, Pierre
%A Limoges, Benoît
%D 2001
%T Gold Nanoparticle-Based Quantitative
Electrochemical Detection of Amplified Human
Cytomegalovirus DNA Using Disposable Microband
Electrodes
%U https://acs.figshare.com/articles/journal_contribution/Gold_Nanoparticle-Based_Quantitative_Electrochemical_Detection_of_Amplified_Human_Cytomegalovirus_DNA_Using_Disposable_Microband_Electrodes/3582690
%R 10.1021/ac0103221.s001
%2 https://ndownloader.figshare.com/files/5668569
%K nanoparticle probe
%K Amplified Human Cytomegalovirus DNA
%K nonlinear diffusion
%K III mass transfer
%K gold nanoparticle probe
%K 5 pM
%K cytomegalovirus DNA sequence
%K SPMBE
%K oxidative metal dissolution
%K Disposable Microband Electrodes
%K III ions
%K HCMV DNA
%K electrochemical DNA detection method
%K electrodeposition time
%K HCMV DNA fragment
%K III determination
%K gold metal atoms
%X An electrochemical DNA detection method has been
developed for the sensitive quantification of an amplified
406-base pair human cytomegalovirus DNA sequence
(HCMV DNA). The assay relies on (i) the hybridization of
the single-stranded target HCMV DNA with an oligonucleotide-modified Au nanoparticle probe, (ii) followed by the
release of the gold metal atoms anchored on the hybrids
by oxidative metal dissolution, and (iii) the indirect
determination of the solubilized AuIII ions by anodic
stripping voltammetry at a sandwich-type screen-printed
microband electrode (SPMBE). Due to the enhancement
of the AuIII mass transfer by nonlinear diffusion during
the electrodeposition time, the SPMBE allows the sensitive determination of AuIII in a small volume of quiescent
solution. The combination of the sensitive AuIII determination at a SPMBE with the large number of AuIII released
from each gold nanoparticle probe allows detection of as
low as 5 pM amplified HCMV DNA fragment.
%I ACS Publications