10.1371/journal.pone.0029948.g004
Aaron Alt
Aaron
Alt
Laura Miguel-Romero
Laura
Miguel-Romero
Jordi Donderis
Jordi
Donderis
Mikel Aristorena
Mikel
Aristorena
Francisco J. Blanco
Francisco J.
Blanco
Adam Round
Adam
Round
Vicente Rubio
Vicente
Rubio
Carmelo Bernabeu
Carmelo
Bernabeu
Alberto Marina
Alberto
Marina
SAXS data analysis and rigid body modeling.
Public Library of Science
2012
rigid
2012-02-08 01:35:55
Figure
https://plos.figshare.com/articles/figure/_SAXS_data_analysis_and_rigid_body_modeling_/355755
<p>(A) The experimental SAXS profile of recombinant Endo<sub>338</sub>, measured at the ID14-3 BioSAXS beamline. The Kratky plot (inserted), displays a gaussian behavior, which indicates the well folded nature of the construct. (B) The distance distribution function, p(r) obtained from GNOM with a D<sub>max</sub> of 9.4 nm also suggests a compact structure. (C) The orphan domain model obtained from I-TASSER together with 5 Man<sub>5</sub>NAG<sub>2</sub> glycans, restricted by their attachment sites was fitted by rigid body modeling with SASREF, yielding a fit of the model (red line) against the experimental Endo<sub>338</sub> scattering profile (blue crosses) of χ<sup>2</sup> = 1.1. The input models used, are depicted above the fit curve. (D) The resulting model is displayed as a cartoon in three orthogonal views, with the attached sugars depicted as balls, fitted into the molecular envelope from DAMMIF.</p>