10.6084/m9.figshare.35564.v1 Satoko Adachi Satoko Adachi Ellen V. Rothenberg Ellen V. Rothenberg Dimethylated histone H3/K4 association with 5′-flanking region is correlated with IL-2 inducibility in immature thymocytes: ChIP analysis of the locus in thymocytes from SCID and MHC-deficient mice figshare 2011 dimethylated mhcdeficient scid locus analysis chip thymocytes immature inducibility correlated region association histone mice 2011-12-31 00:01:42 Figure https://figshare.com/articles/figure/Dimethylated_histone_H3_K4_association_with_5_flanking_region_is_correlated_with_IL_2_inducibility_in_immature_thymocytes_ChIP_analysis_of_the_locus_in_thymocytes_from_SCID_and_MHC_deficient_mice/35564 <p><b>Copyright information:</b></p><p>Taken from "Cell-type-specific epigenetic marking of the gene at a distal -regulatory region in competent, nontranscribing T-cells"</p><p>Nucleic Acids Research 2005;33(10):3200-3210.</p><p>Published online 3 Jun 2005</p><p>PMCID:PMC1142491.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Cells from MHC-deficient mice, >95% CD4 CD8 TCR-low, were cultured with or without PMA/A23187 for 3 h. Cells from SCID mice, >95% CD4 CD8 TCR-negative, were cultured with or without stimulation for 2 h, and IL-1α was also added as indicated. To correct for differences in efficiency of immune precipitation between these samples due to the extensive differences in cell physiology, ChIP analysis results for diMeH3/K4 at the locus for each sample were normalized by results at the S16 ribosomal protein locus. Two independent experiments were done with similar results.