Pécastaings, Sophie Allombert, Julie Lajoie, Barbora Doublet, Patricia Roques, Christine Vianney, Anne New insights into <i>Legionella pneumophila</i> biofilm regulation by c-di-GMP signaling <p>The waterborne pathogen <i>Legionella pneumophila</i> grows as a biofilm, freely or inside amoebae. Cyclic-di-GMP (c-di-GMP), a bacterial second messenger frequently implicated in biofilm formation, is synthesized and degraded by diguanylate cyclases (DGCs) and phosphodiesterases (PDEs), respectively. To characterize the c-di-GMP-metabolizing enzymes involved in <i>L. pneumophila</i> biofilm regulation, the consequences on biofilm formation and the c-di-GMP concentration of each corresponding gene inactivation were assessed in the Lens strain. The results showed that one DGC and two PDEs enhance different aspects of biofilm formation, while two proteins with dual activity (DGC/PDE) inhibit biofilm growth. Surprisingly, only two mutants exhibited a change in global c-di-GMP concentration. This study highlights that specific c-di-GMP pathways control <i>L. pneumophila</i> biofilm formation, most likely <i>via</i> temporary and/or local modulation of c-di-GMP concentration. Furthermore, Lpl1054 DGC is required to enable the formation a dense biofilm in response to nitric oxide, a signal for biofilm dispersion in many other species.</p> Biofilm;Legionella;c-di-GMP signaling;second messenger;NO response 2016-08-05
    https://tandf.figshare.com/articles/journal_contribution/New_insights_into_i_Legionella_pneumophila_i_biofilm_regulation_by_c-di-GMP_signaling/3543197
10.6084/m9.figshare.3543197.v1