10.1371/journal.pone.0032287.g004
Hans J. Rolf
Hans
J. Rolf
Sabine Niebert
Sabine
Niebert
Marcus Niebert
Marcus
Niebert
Lena Gaus
Lena
Gaus
Henning Schliephake
Henning
Schliephake
K. Günter Wiese
K.
Günter Wiese
Cytoskeletal elements of STRO-1<sup>+</sup> DaMSCs and long-distance cell-to-cell connections (TNTs).
Public Library of Science
2012
elements
damscs
long-distance
cell-to-cell
connections
2012-02-16 00:45:55
Figure
https://plos.figshare.com/articles/figure/_Cytoskeletal_elements_of_STRO_1_DaMSCs_and_long_distance_cell_to_cell_connections_TNTs_/352755
<p>(<b>a,b</b>) Different cell-to-cell connections between STRO-1<sup>+</sup> cells. Intercellular connections are able to bridge long distances even across neighbouring cells (Phalloidin/Tubulin staining, merged images). (<b>b</b>) Thick tubes (diameter >0.4 µm, example is marked by red arrow) contain F-actin and α-tubulin, whereas thin tubes (diameter <0.4 µm, example is marked by green arrow) contain only F-actin. (<b>c</b>) Negative control, staining without Tubulin antibody. (<b>d–f</b>) Scanning electron microscope pictures (SEM) of a mixed culture of antlerogenic cells. Cells form long connections across neighbouring cells (<b>d,e</b>) and very high magnification (<b>f</b>) proves that the surface of TNTs exhibit small appendages; long-distance connections are also possible between morphologically distinguishable cell types (<b>d</b>). (<b>h–m</b>) Multichannel pictures of Phalloidin (<b>h,k</b>) and Tubulin (<b>i,l</b>) stained DaMSCs demonstrate that their intercellular connections continuously consist of F-actin but only partially of microtubules. The visible spindle apparatus (<b>i</b>, detail enlargement) point to an initiating cell division and provides evidence that the used α-tubulin antibody is also efficient in DaMSCs. (<b>j,m</b>). Merged Images.</p>