10.1371/journal.pone.0032197.g004
Olivier Moralès
Olivier
Moralès
Audrey Richard
Audrey
Richard
Nathalie Martin
Nathalie
Martin
Dhafer Mrizak
Dhafer
Mrizak
Magalie Sénéchal
Magalie
Sénéchal
Céline Miroux
Céline
Miroux
Véronique Pancré
Véronique
Pancré
Jean Rommelaere
Jean
Rommelaere
Perrine Caillet-Fauquet
Perrine
Caillet-Fauquet
Yvan de Launoit
Yvan
de Launoit
Nadira Delhem
Nadira
Delhem
Unlike PBMCs, CD4+ T cells do not undergo any major impairment upon H-1 PV infection.
Public Library of Science
2013
cells
impairment
h-1
pv
2013-02-20 09:00:40
Figure
https://plos.figshare.com/articles/figure/_Unlike_PBMCs_CD4_T_cells_do_not_undergo_any_major_impairment_upon_H_1_PV_infection_/352753
<p>CD4+ T cells were inoculated with increasing amounts of H-1 PV or mock-treated. MOI : multiplicity of infection, expressed as the number of plate-forming unit/cell; p.i : post-infection. <b>A</b>. <i>CD4+ T cell proliferation ability is not altered upon H-1 PV infection</i>. Cell proliferation was assessed by metabolic incorporation of tritiated thymidine into cellular DNA. Only 48 and 72 h p.i conditions are shown. Results are represented as means of triplicate wells with ± standard deviation bars and expressed in count per minute (cpm). <b>B</b>. <i>H-1 PV does not affect CD4+ T cell viability</i>. Cell viability was evaluated using a test based on a bioluminescent reaction measuring the amount of ATP present in living cells. Only 48 and 72 h p.i conditions are shown. Results are represented as means of triplicate wells with ± standard deviation bars and expressed in relative light unit (RLU). <b>C</b>. <i>Infection of CD4 T cells by H-1 PV is not related to any morphological changes</i>. For each condition, pictures of cells were taken in the course of infection but only those taken 48 h after H-1 PV inoculation are shown. Scale bar = 100 µm. <b>D</b>. <i>H-1 PV does not modify CD4+ T cell basal lysis</i>. H-1 PV cytotoxicity was assessed using a test based on a bioluminescent reaction measuring the leak of a cellular marker from cytoplasm to culture medium, which reveals the loss of plasma membrane integrity. Results are represented as means of triplicate wells with ± standard deviation bars and expressed in relative light unit (RLU). <b>E</b>. <i>CD4+ T cells transiently express a slight amount of NS1 protei</i>n. Equal amounts of total CD4+ T cell proteins were separated by 4–12% SDS-PAGE and analyzed by Western blot for the presence of NS1 protein. Erk2 was used as a loading control. <i>Representative data from 2 independent experiments</i>.</p>