10.1371/journal.pone.0031361.g003 Galyna Gergalova Galyna Gergalova Olena Lykhmus Olena Lykhmus Olena Kalashnyk Olena Kalashnyk Lyudmyla Koval Lyudmyla Koval Volodymyr Chernyshov Volodymyr Chernyshov Elena Kryukova Elena Kryukova Victor Tsetlin Victor Tsetlin Sergiy Komisarenko Sergiy Komisarenko Maryna Skok Maryna Skok The effects of α7-specific ligands and DIDS on Ca<sup>2+</sup> accumulation in mitochondria studied by flow cytometry. Public Library of Science 2013 ligands dids accumulation mitochondria studied 2013-02-20 08:54:28 Figure https://plos.figshare.com/articles/figure/_The_effects_of_7_specific_ligands_and_DIDS_on_Ca_2_accumulation_in_mitochondria_studied_by_flow_cytometry_/351588 <p><b>A</b> – purified liver mitochondria gated by size (Forward scatter) and granularity (Side scatter) in flow cytometry. <b>B</b> – binding of 0.1 µM acridine orange 10-nonyl bromide (NAO) to the gated mitochondria population; Control – the non-stained mitochondria. <b>C</b> – Ca<sup>2+</sup> accumulation in mitochondria loaded with Fluo 3-AM. <b>D</b> – Ca<sup>2+</sup> accumulated in mitochondria during 2 min after 1 min pretreatment with DIDS, acetylcholine, choline, PNU-282987 or MLA; data are shown as normalized mean fluorescence values of 5 independent experiments for each ligand; * – <i>P<0.05</i> compared to the fluorescence in the absence of α7 nAChR agonists or DIDS.</p>