10.1371/journal.pone.0031361.g003
Galyna Gergalova
Galyna
Gergalova
Olena Lykhmus
Olena
Lykhmus
Olena Kalashnyk
Olena
Kalashnyk
Lyudmyla Koval
Lyudmyla
Koval
Volodymyr Chernyshov
Volodymyr
Chernyshov
Elena Kryukova
Elena
Kryukova
Victor Tsetlin
Victor
Tsetlin
Sergiy Komisarenko
Sergiy
Komisarenko
Maryna Skok
Maryna
Skok
The effects of α7-specific ligands and DIDS on Ca<sup>2+</sup> accumulation in mitochondria studied by flow cytometry.
Public Library of Science
2013
ligands
dids
accumulation
mitochondria
studied
2013-02-20 08:54:28
Figure
https://plos.figshare.com/articles/figure/_The_effects_of_7_specific_ligands_and_DIDS_on_Ca_2_accumulation_in_mitochondria_studied_by_flow_cytometry_/351588
<p><b>A</b> – purified liver mitochondria gated by size (Forward scatter) and granularity (Side scatter) in flow cytometry. <b>B</b> – binding of 0.1 µM acridine orange 10-nonyl bromide (NAO) to the gated mitochondria population; Control – the non-stained mitochondria. <b>C</b> – Ca<sup>2+</sup> accumulation in mitochondria loaded with Fluo 3-AM. <b>D</b> – Ca<sup>2+</sup> accumulated in mitochondria during 2 min after 1 min pretreatment with DIDS, acetylcholine, choline, PNU-282987 or MLA; data are shown as normalized mean fluorescence values of 5 independent experiments for each ligand; * – <i>P<0.05</i> compared to the fluorescence in the absence of α7 nAChR agonists or DIDS.</p>