10.6084/m9.figshare.3503852.v1
Ajit Kumar Mahapatra
Ajit Kumar
Mahapatra
Rajkishor Maji
Rajkishor
Maji
Kalipada Maiti
Kalipada
Maiti
Sanchita Mondal
Sanchita
Mondal
Syed Samim Ali
Syed
Samim Ali
Saikat Kumar Manna
Saikat
Kumar Manna
Prithidipa Sahoo
Prithidipa
Sahoo
Carbazole-driven ratiometric fluorescence turn on for dual ion recognition of Zn<sup>2+</sup> and Hg<sup>2+</sup> by thiophene-pyridyl conjugate in HEPES buffer medium: spectroscopy, computational, microscopy and cellular studies
Taylor & Francis Group
2016
Ratiometric fluorescent probe
zinc and mercury ions
single-crystal X-ray diffraction
chemoensembles
living cells
2016-07-28 08:28:27
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Carbazole-driven_ratiometric_fluorescence_turn_on_for_dual_ion_recognition_of_Zn_sup_2_sup_and_Hg_sup_2_sup_by_thiophene-pyridyl_conjugate_in_HEPES_buffer_medium_spectroscopy_computational_microscopy_and_cellular_studies/3503852
<p>Carbazole-based thiophene-pyridyl conjugate (<b>L</b>) was synthesised and characterised. The complexation between <b>L</b> with Zn<sup>2+</sup> and Hg<sup>2+</sup> was studied in HEPES buffer medium by fluorescence, absorption and visual colour change with the detection limit of ~3.7 and ~4.8 μM, respectively. The <b>L</b> detects Zn<sup>2+</sup> by bringing ratiometric change in the fluorescence signals at 418 and 515 nm, but in the case of Hg<sup>2+</sup>, the signals are observed at 418 and 365 nm, while no new band is observed at 515 nm. The structure of <b>L</b> has been established by single-crystal XRD and that of complexes <b>[ZnL]</b> and <b>[HgL]</b> by density functional theory calculations. TDDFT calculations were performed in order to demonstrate the electronic properties of receptors and their zinc and mercury complexes. The isolated fluorescent complexes <b>[ZnL]</b> and <b>[HgL]</b> were found to be sensitive and selective towards phosphate-bearing ions and sulfide ions, respectively, among the other anions studied. The nanostructural features such as shape and size obtained using atomic force microscopy distinguish <b>L</b> from its complexes formed between <b>L</b> and Zn<sup>2+</sup> from that formed with Hg<sup>2+</sup>. Moreover, the utility of the conjugate <b>L</b> in showing the zinc recognition in live cells has also been demonstrated using RAW cells as monitored by fluorescence imaging.</p>