10.1371/journal.pone.0031848.g005 Naomi W. Lucchi Naomi W. Lucchi Mitra Poorak Mitra Poorak Jenna Oberstaller Jenna Oberstaller Jeremy DeBarry Jeremy DeBarry Ganesh Srinivasamoorthy Ganesh Srinivasamoorthy Ira Goldman Ira Goldman Maniphet Xayavong Maniphet Xayavong Alexandre J. da Silva Alexandre J. da Silva David S. Peterson David S. Peterson John W. Barnwell John W. Barnwell Jessica Kissinger Jessica Kissinger Venkatachalam Udhayakumar Venkatachalam Udhayakumar Limits of detection of primer set Pkr140-5. Public Library of Science 2013 detection primer 2013-02-20 08:47:41 Figure https://plos.figshare.com/articles/figure/_Limits_of_detection_of_primer_set_Pkr140_5_/350192 <p>The analytical sensitivity of primer set Pk140-5 (A) and the primers from a published study <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031848#pone.0031848-Singh1" target="_blank">[3]</a> (B) were determined using a well-quantitated <i>P. knowlesi</i> DNA standard. The blood sample was serially diluted ten-fold with a starting parasitemia of 100,000p/µl to 1p/µl. The expected base pair sizes for the two primers are included. Three different experiments are shown.</p>