TY - DATA T1 - Supplementary Material for: Transport and Catabolism of Carbohydrates by Neisseria meningitidis PY - 2016/07/25 AU - Derkaoui M. AU - Antunes A. AU - Nait Abdallah J. AU - Poncet S. AU - Mazé A. AU - Ma Pham Q.M. AU - Mokhtari A. AU - Deghmane A.-E. AU - Joyet P. AU - Taha M.-K. AU - Deutscher J. UR - https://karger.figshare.com/articles/figure/Supplementary_Material_for_Transport_and_Catabolism_of_Carbohydrates_by_Neisseria_meningitidis/3497855 DO - 10.6084/m9.figshare.3497855.v1 L4 - https://ndownloader.figshare.com/files/5527592 KW - Neisseria meningitidis KW - Glucose KW - Maltose KW - Gluconate KW - Virulence N2 - We identified the genes encoding the proteins for the transport of glucose and maltose in Neisseria meningitidis strain 2C4-3. A mutant deleted for NMV_1892(glcP)no longer grew on glucose and deletion of NMV_0424(malY) prevented the utilization of maltose. We also purified and characterized glucokinase and α-phosphoglucomutase, which catalyze early catabolic steps of the two carbohydrates.N. meningitidis catabolizes the two carbohydrates either via the Entner-Doudoroff (ED) pathway or the pentose phosphate pathway, thereby forming glyceraldehyde-3-P and either pyruvate or fructose-6-P, respectively. We purified and characterized several key enzymes of the two pathways. The genes required for the transformation of glucose into gluconate-6-P and its further catabolism via the ED pathway are organized in two adjacent operons. N. meningitidis also contains genes encoding proteins which exhibit similarity to the gluconate transporter (NMV_2230) and gluconate kinase (NMV_2231) of Enterobacteriaceae and Firmicutes. However, gluconate might not be the real substrate of NMV_2230 because N. meningitidis was not able to grow on gluconate as the sole carbon source. Surprisingly, deletion ofNMV_2230 stimulated growth in minimal medium in the presence and absence of glucose and drastically slowed the clearance of N. meningitidis cells from transgenic mice after intraperitoneal challenge. ER -