Supplementary Material for: Induced Disruption of the Iron-Regulatory Hormone Hepcidin Inhibits Acute Inflammatory Hypoferraemia
Armitage A.E.
Lim P.J.
Frost J.N.
Pasricha S.-R.
Soilleux E.J.
Evans E.
Morovat A
Santos A.
Diaz R.
Biggs D.
Davies B.
Gileadi U.
Robbins P.A.
Lakhal-Littleton S.
Drakesmith H.
10.6084/m9.figshare.3490304.v1
https://karger.figshare.com/articles/figure/Supplementary_Material_for_Induced_Disruption_of_the_Iron-Regulatory_Hormone_Hepcidin_Inhibits_Acute_Inflammatory_Hypoferraemia/3490304
Withdrawal of iron from serum (hypoferraemia) is a conserved innate immune antimicrobial strategy that can withhold this critical nutrient from invading pathogens, impairing their growth. Hepcidin <i>(Hamp1)</i> is the master regulator of iron and its expression is induced by inflammation. Mice lacking <i>Hamp1</i> from birth rapidly accumulate iron and are susceptible to infection by blood-dwelling siderophilic bacteria such as <i>Vibrio vulnificus</i>. In order to study the innate immune role of hepcidin against a background of normal iron status, we developed a transgenic mouse model of tamoxifen-sensitive conditional <i>Hamp1</i> deletion (termed i<i>Hamp1</i>-KO mice). These mice attain adulthood with an iron status indistinguishable from littermate controls.<i>Hamp1</i> disruption and the consequent decline of serum hepcidin concentrations occurred within hours of a single tamoxifen dose. We found that the TLR ligands LPS and Pam3CSK4 and heat-killed <i>Brucella abortus</i> caused an equivalent induction of inflammation in control and i<i>Hamp1</i>-KO mice. Pam3CSK4 and <i>B. abortus</i> only caused a drop in serum iron in control mice, while hypoferraemia due to LPS was evident but substantially blunted in i<i>Hamp1</i>-KO mice<i>. </i>Our results characterise a powerful new model of rapidly inducible hepcidin disruption, and demonstrate the critical contribution of hepcidin to the hypoferraemia of inflammation.
2016-07-18 06:06:00
Iron
Inflammation
Hepcidin
Hypoferraemia