SOP - DNA Isolation QIAamp Fast DNA Stool Modified
Berith E. Knudsen
Lasse Bergmark
Sünje Johanna Pamp
10.6084/m9.figshare.3475406.v4
https://figshare.com/articles/journal_contribution/SOP_-_DNA_Isolation_QIAamp_Fast_DNA_Stool_Modified/3475406
This protocol for DNA isolation is based on the Qiagen kit QIAamp Fast DNA stool mini kit (cat. no. 51604). The method has been tested using different sample matrices, e.g. human and animal feces and sewage. The protocol “Isolation of DNA from Stool for Pathogen Detection” supplied with the kit has been modified in order to increase the DNA yield from these complex samples while maintaining high quality of the DNA. The main modification to the protocol supplied with the kit is the addition of a bead-beating step in the beginning of the isolation to facilitate increasing cell lysis, e.g. of Gram-positive bacteria. Additional modifications have been included as described herein. For example, for the extraction of DNA from sewage specimens a few additional steps are included to further increase the DNA yield.<br>
<p><b>If you use this method, please cite</b>:</p>
<p>Berith E. Knudsen, Lasse Bergmark, Patrick Munk,
Oksana Lukjancenko, Anders Priemé, Frank M. Aarestrup, Sünje J. Pamp (2016) Impact of Sample
Type and DNA Isolation Procedure on Genomic Inference of Microbiome
Composition. <b><i>mSystems</i></b> Oct 2016, 1 (5) e00095-16; DOI:
10.1128/mSystems.00095-16</p>
<p><a href="http://msystems.asm.org/content/1/5/e00095-16">http://msystems.asm.org/content/1/5/e00095-16</a><u></u></p>
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2016-10-18 16:26:23
DNA extraction
microbiome
genomics
metagenomics
16S rRNA gene profiling
human
pig
sewage
fecal sample
Microbial community compositions
mock community
spiking
Qiagen
DNA
Protocol
Standard operating procedure
Bacteriology
Biogeography and Phylogeography
Bioinformatics
Biological Techniques
Biotechnology
Community Ecology (excl. Invasive Species Ecology)
Computational Biology
Evolutionary Biology
Forensic Biology
Freshwater Ecology
Genomics
Host-Parasite Interactions
Infectious Agents
Infectious Diseases
Microbial Ecology
Microbial Genetics
Microbiology
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