SOP - DNA Isolation QIAamp Fast DNA Stool Modified Berith E. Knudsen Lasse Bergmark Sünje Johanna Pamp 10.6084/m9.figshare.3475406.v4 https://figshare.com/articles/journal_contribution/SOP_-_DNA_Isolation_QIAamp_Fast_DNA_Stool_Modified/3475406 This protocol for DNA isolation is based on the Qiagen kit QIAamp Fast DNA stool mini kit (cat. no. 51604). The method has been tested using different sample matrices, e.g. human and animal feces and sewage. The protocol “Isolation of DNA from Stool for Pathogen Detection” supplied with the kit has been modified in order to increase the DNA yield from these complex samples while maintaining high quality of the DNA. The main modification to the protocol supplied with the kit is the addition of a bead-beating step in the beginning of the isolation to facilitate increasing cell lysis, e.g. of Gram-positive bacteria. Additional modifications have been included as described herein. For example, for the extraction of DNA from sewage specimens a few additional steps are included to further increase the DNA yield.<br> <p><b>If you use this method, please cite</b>:</p> <p>Berith E. Knudsen, Lasse Bergmark, Patrick Munk, Oksana Lukjancenko, Anders Priemé, Frank M. Aarestrup, Sünje J. Pamp (2016) Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition. <b><i>mSystems</i></b> Oct 2016, 1 (5) e00095-16; DOI: 10.1128/mSystems.00095-16</p> <p><a href="http://msystems.asm.org/content/1/5/e00095-16">http://msystems.asm.org/content/1/5/e00095-16</a><u></u></p> <br><br><br> 2016-10-18 16:26:23 DNA extraction microbiome genomics metagenomics 16S rRNA gene profiling human pig sewage fecal sample Microbial community compositions mock community spiking Qiagen DNA Protocol Standard operating procedure Bacteriology Biogeography and Phylogeography Bioinformatics Biological Techniques Biotechnology Community Ecology (excl. Invasive Species Ecology) Computational Biology Evolutionary Biology Forensic Biology Freshwater Ecology Genomics Host-Parasite Interactions Infectious Agents Infectious Diseases Microbial Ecology Microbial Genetics Microbiology Molecular Biology Molecular Evolution Parasitology Population Ecology Systems Biology