10.6084/m9.figshare.3473567.v2 Micaela Tamara Vitor Micaela Tamara Vitor Patrícia Cruz Bergami-Santos Patrícia Cruz Bergami-Santos Rafael Henrique Freitas Zômpero Rafael Henrique Freitas Zômpero Karen Steponavicius Piedade Cruz Karen Steponavicius Piedade Cruz Mariana Pereira Pinho Mariana Pereira Pinho José Alexandre Marzagão Barbuto José Alexandre Marzagão Barbuto Lucimara Gaziola de la Torre Lucimara Gaziola de la Torre Cationic liposomes produced via ethanol injection method for dendritic cell therapy Taylor & Francis Group 2017 T lymphocyte proliferation ethanol injection method costimulatory molecule CD 86 DOTAP cationic liposomes gene delivery system EPC MFV size reduction processes DSC TEM transmission electron microscopy VEI ST DC dendritic cell therapy Cationic liposomes nm DOPE 2017-11-03 06:49:44 Dataset https://tandf.figshare.com/articles/dataset/Cationic_liposomes_produced_via_ethanol_injection_method_for_dendritic_cell_therapy/3473567 <p>Cationic liposomes can be designed and developed in order to be an efficient gene delivery system for mammalian cells. Dendritic cell (DC) vaccines can be used to treat cancer, as cationic liposomes can deliver tumor antigens to cells while cells remain active. However, most methods used for liposome production are not able to reproduce in large scale the physicochemical and biological properties of liposomes produced in laboratory scale. In this context, ethanol injection method achieved promising results, although requiring post-treatment for size reduction and/or to remove residual ethanol. Thus, the purpose of this study was to generate cationic liposomes suitable for gene therapies via ethanol injection method in only one step (VEI) and compared to those submitted to a size reduction processes by microfluidization (MFV). For this, the method to produce cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and 1,2-dioleoylphosphatidylethanolamine (DOPE) was optimized using a statistical design approach. As a result, the size of VEI decreased from 290 nm to 110 nm and the polydispersity from 0.54 to 0.17. In the case of MFV, size decreased from 128 nm to 107 nm and polydispersity from 0.40 to 0.18. ST and MFV before and after optimization were also characterized in terms of morphology by transmission electron microscopy (TEM) and structure by differential scanning calorimetry (DSC). Finally, to show their potential in gene/immune therapies applications, DCs were stimulated by such liposomes. Cells internalized liposomes, increasing expression of the costimulatory molecule CD86 and inducing T lymphocyte proliferation.</p>