Kobayashi, Taiki Sasaki, Satoshi Utsumi, Yoshinori Fujita, Naoko Umeda, Kazuhiro Sawada, Takayuki Kubo, Akiko Abe, Jun-ichi Colleoni, Christophe Ball, Steven Nakamura, Yasunori Comparison of Chain-Length Preferences and Glucan Specificities of Isoamylase-Type α-Glucan Debranching Enzymes from Rice, Cyanobacteria, and Bacteria <div><p>It has been believed that isoamylase (ISA)-type α-glucan debranching enzymes (DBEs) play crucial roles not only in α-glucan degradation but also in the biosynthesis by affecting the structure of glucans, although molecular basis on distinct roles of the individual DBEs has not fully understood. In an attempt to relate the roles of DBEs to their chain-length specificities, we analyzed the chain-length distribution of DBE enzymatic reaction products by using purified DBEs from various sources including rice, cyanobacteria, and bacteria. When DBEs were incubated with phytoglycogen, their chain-length specificities were divided into three groups. First, rice endosperm ISA3 (OsISA3) and <i>Eschericia coli</i> GlgX (EcoGlgX) almost exclusively debranched chains having degree of polymerization (DP) of 3 and 4. Second, OsISA1, <i>Pseudomonas amyloderamosa</i> ISA (PsaISA), and rice pullulanase (OsPUL) could debranch a wide range of chains of DP≧3. Third, both cyanobacteria ISAs, <i>Cyanothece</i> ATCC 51142 ISA (CytISA) and <i>Synechococcus elongatus</i> PCC7942 ISA (ScoISA), showed the intermediate chain-length preference, because they removed chains of mainly DP3-4 and DP3-6, respectively, while they could also react to chains of DP5-10 and 7–13 to some extent, respectively. In contrast, all these ISAs were reactive to various chains when incubated with amylopectin. In addition to a great variation in chain-length preferences among various ISAs, their activities greatly differed depending on a variety of glucans. Most strikingly, cyannobacteria ISAs could attack branch points of pullulan to a lesser extent although no such activity was found in OsISA1, OsISA3, EcoGlgX, and PsaISA. Thus, the present study shows the high possibility that varied chain-length specificities of ISA-type DBEs among sources and isozymes are responsible for their distinct functions in glucan metabolism.</p></div> Synechococcus elongatus PCC 7942 ISA;DBE;cyanobacteria ISAs;rice pullulanase;Glucan Specificities;rice endosperm ISA 3;branch points;Eschericia coli GlgX;OsISA;glucan metabolism;cyannobacteria ISAs;debranched chains;DP;reaction products;Cyanothece ATCC 51142 ISA 2016-06-16
    https://plos.figshare.com/articles/dataset/Comparison_of_Chain-Length_Preferences_and_Glucan_Specificities_of_Isoamylase-Type_-Glucan_Debranching_Enzymes_from_Rice_Cyanobacteria_and_Bacteria/3449270
10.1371/journal.pone.0157020