TY - DATA T1 - Determination of fate of CCR2 downregulation on monocytes from Ficoll. PY - 2013/02/20 AU - Juan C. Nieto AU - Elisabet Cantó AU - Carlos Zamora AU - M. Angels Ortiz AU - Cándido Juárez AU - Silvia Vidal UR - https://plos.figshare.com/articles/figure/_Determination_of_fate_of_CCR2_downregulation_on_monocytes_from_Ficoll_/342574 DO - 10.1371/journal.pone.0031297.g004 L4 - https://ndownloader.figshare.com/files/672088 KW - ccr2 KW - downregulation KW - monocytes N2 - CCR2 expression was assessed after Ficoll isolation of untagged and tagged-CCR2 monocytes. Internalization of the complex was examined from one single layer of Z stacks by confocal microscopy. The results of a representative experiment are shown: a) CCR2 expression on monocytes from WB tagged with CCR2 prior to gradient separation; b) CCR2 expression on untagged monocytes after Ficoll isolation and the subsequent staining of CCR2; c) CCR2 expression in CCR2 tagged monocytes after Ficoll isolation, red signal within monocytes represents the CCR2-complex internalization (no aditional anti CCR2-PE was added to the cells after isolation with Ficoll); (isotype control □ , chemokine receptor ▪). ER -