Complementation of <i>bh0463A</i> in a <i>dam</i><sup>-</sup> <i>Escherichia coli</i> strain results in adenine methylation of plasmid DNA. JamesAllison E. S. RogovskyyArtem CrowleyMichael A. BankheadTroy 2016 <p>Control plasmid pAE30 (lanes 1, 3, 5, and 7) and a <i>bh0463A</i> complementation plasmid (pAE35; lanes 2, 4, 6, and 8) were extracted from a <i>dam</i><sup>-</sup> <i>E</i>. <i>coli</i> strain and subjected to digestion with DpnI (lanes 3 and 4), MboI (lanes 5 and 6), or Sau3AI (lanes 7 and 8). DpnI, MboI, and Sau3A1 cut GATC sequences that are adenine methylated, adenine non-methylated, or cytosine non-methylated, respectively. Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs.</p>