10.1371/journal.pone.0155798.g001
Allison E. James
Allison E.
James
Artem S. Rogovskyy
Artem
S. Rogovskyy
Michael A. Crowley
Michael A.
Crowley
Troy Bankhead
Troy
Bankhead
Complementation of <i>bh0463A</i> in a <i>dam</i><sup>-</sup> <i>Escherichia coli</i> strain results in adenine methylation of plasmid DNA.
Public Library of Science
2016
bh 0463A
hermsii viability
infectivity
coli
immunocompetent mice
locus bh 0463A encodes
persistence
Dam
DNA methyltransferase gene
Persistence DNA methyltransferases
Relapsing fever Borrelia species harbor
Restriction fragment analysis
Lyme disease agent
DNA adenine methyltransferase
dam
DNA Adenine Methyltransferase Gene
BH 0463A protein product
relapsing fever species Borrelia hermsii
2016-05-19 10:04:24
Figure
https://plos.figshare.com/articles/figure/Complementation_of_i_bh0463A_i_in_a_i_dam_i_sup_-_sup_i_Escherichia_coli_i_strain_results_in_adenine_methylation_of_plasmid_DNA_/3391009
<p>Control plasmid pAE30 (lanes 1, 3, 5, and 7) and a <i>bh0463A</i> complementation plasmid (pAE35; lanes 2, 4, 6, and 8) were extracted from a <i>dam</i><sup>-</sup> <i>E</i>. <i>coli</i> strain and subjected to digestion with DpnI (lanes 3 and 4), MboI (lanes 5 and 6), or Sau3AI (lanes 7 and 8). DpnI, MboI, and Sau3A1 cut GATC sequences that are adenine methylated, adenine non-methylated, or cytosine non-methylated, respectively. Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs.</p>