10.1371/journal.pone.0155798.g001 Allison E. James Allison E. James Artem S. Rogovskyy Artem S. Rogovskyy Michael A. Crowley Michael A. Crowley Troy Bankhead Troy Bankhead Complementation of <i>bh0463A</i> in a <i>dam</i><sup>-</sup> <i>Escherichia coli</i> strain results in adenine methylation of plasmid DNA. Public Library of Science 2016 bh 0463A hermsii viability infectivity coli immunocompetent mice locus bh 0463A encodes persistence Dam DNA methyltransferase gene Persistence DNA methyltransferases Relapsing fever Borrelia species harbor Restriction fragment analysis Lyme disease agent DNA adenine methyltransferase dam DNA Adenine Methyltransferase Gene BH 0463A protein product relapsing fever species Borrelia hermsii 2016-05-19 10:04:24 Figure https://plos.figshare.com/articles/figure/Complementation_of_i_bh0463A_i_in_a_i_dam_i_sup_-_sup_i_Escherichia_coli_i_strain_results_in_adenine_methylation_of_plasmid_DNA_/3391009 <p>Control plasmid pAE30 (lanes 1, 3, 5, and 7) and a <i>bh0463A</i> complementation plasmid (pAE35; lanes 2, 4, 6, and 8) were extracted from a <i>dam</i><sup>-</sup> <i>E</i>. <i>coli</i> strain and subjected to digestion with DpnI (lanes 3 and 4), MboI (lanes 5 and 6), or Sau3AI (lanes 7 and 8). DpnI, MboI, and Sau3A1 cut GATC sequences that are adenine methylated, adenine non-methylated, or cytosine non-methylated, respectively. Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs.</p>