TY - DATA T1 - Ofd1, ciliary associated genes and Wnt signaling pathway genes expression in RCS rat retina. PY - 2016/05/19 AU - Juan Wang AU - Xin Chen AU - Fang Wang AU - Jieping Zhang AU - Peng Li AU - Zongyi Li AU - Jingying Xu AU - Furong Gao AU - Caixia Jin AU - Haibin Tian AU - Jingfa Zhang AU - Weiye Li AU - Lixia Lu AU - Guo-Tong Xu UR - https://plos.figshare.com/articles/figure/Ofd1_ciliary_associated_genes_and_Wnt_signaling_pathway_genes_expression_in_RCS_rat_retina_/3390895 DO - 10.1371/journal.pone.0155860.g002 L4 - https://ndownloader.figshare.com/files/5298037 KW - RP KW - ofd 1 neuroprotection KW - Reactive oxygen species KW - Apoptosis genes expression KW - R 28 cells KW - rat retina photoreceptors KW - MNU KW - ROS KW - 1 st KW - Ofd 1 function KW - retina degeneration models KW - Ofd 1 localization KW - photoreceptor degeneration models KW - rat models KW - photoreceptor cilium length KW - Ofd 1 overexpression KW - rat retina KW - Photoreceptor Degeneration Models Ofd 1 KW - RCS KW - OFD KW - ciliary proteins expression levels KW - pathway proteins expression KW - ICC KW - degeneration rat models KW - Ofd 1 KW - Exhibits Neuroprotective Function KW - Ofd 1 expression KW - FACS N2 - RCS rats were sacrificed between 1 and 6 postnatal weeks. One-week-old (1w) RCS rats were used as the control. (A) Ofd1 mRNA expression level increased between the 1st and 4th weeks, and decreased after the 4th week, as determined by qRT-PCR. N = 4. (B) Expression level of Ofd1, Lca5 and Follistatin in RCS rat retina determined using Western blotting analysis. (C) Quantified results of Ofd1, Lca5 and Follistatin protein levels in RCS rats by QuantityOne software. Ofd1 showed the same expression pattern at the mRNA level. Lca5 and Follistatin expression presented the same pattern as Ofd1. (D) Expression level as determined by qRT-PCR of ciliary associated genes Lca5, Fam161a, Rpgrip1, Cep290 and Wnt signaling pathway genes: Axin2 and Follistatin. N = 4. *P<0.05, **P<0.01. For quantification results, three replicates were performed but only one band for three representative samples per group was shown. ER -