Ofd1, ciliary associated genes and Wnt signaling pathway genes expression in RCS rat retina.
Juan Wang
Xin Chen
Fang Wang
Jieping Zhang
Peng Li
Zongyi Li
Jingying Xu
Furong Gao
Caixia Jin
Haibin Tian
Jingfa Zhang
Weiye Li
Lixia Lu
Guo-Tong Xu
10.1371/journal.pone.0155860.g002
https://plos.figshare.com/articles/figure/Ofd1_ciliary_associated_genes_and_Wnt_signaling_pathway_genes_expression_in_RCS_rat_retina_/3390895
<p>RCS rats were sacrificed between 1 and 6 postnatal weeks. One-week-old (1w) RCS rats were used as the control. (A) <i>Ofd1</i> mRNA expression level increased between the 1st and 4th weeks, and decreased after the 4th week, as determined by qRT-PCR. N = 4. (B) Expression level of Ofd1, Lca5 and Follistatin in RCS rat retina determined using Western blotting analysis. (C) Quantified results of Ofd1, Lca5 and Follistatin protein levels in RCS rats by QuantityOne software. Ofd1 showed the same expression pattern at the mRNA level. Lca5 and Follistatin expression presented the same pattern as Ofd1. (D) Expression level as determined by qRT-PCR of ciliary associated genes <i>Lca5</i>, <i>Fam161a</i>, <i>Rpgrip1</i>, <i>Cep290</i> and Wnt signaling pathway genes: <i>Axin2</i> and <i>Follistatin</i>. N = 4. *P<0.05, **P<0.01. For quantification results, three replicates were performed but only one band for three representative samples per group was shown.</p>
2016-05-19 10:01:54
RP
ofd 1 neuroprotection
Reactive oxygen species
Apoptosis genes expression
R 28 cells
rat retina photoreceptors
MNU
ROS
1 st
Ofd 1 function
retina degeneration models
Ofd 1 localization
photoreceptor degeneration models
rat models
photoreceptor cilium length
Ofd 1 overexpression
rat retina
Photoreceptor Degeneration Models Ofd 1
RCS
OFD
ciliary proteins expression levels
pathway proteins expression
ICC
degeneration rat models
Ofd 1
Exhibits Neuroprotective Function
Ofd 1 expression
FACS