Ofd1, ciliary associated genes and Wnt signaling pathway genes expression in RCS rat retina. Juan Wang Xin Chen Fang Wang Jieping Zhang Peng Li Zongyi Li Jingying Xu Furong Gao Caixia Jin Haibin Tian Jingfa Zhang Weiye Li Lixia Lu Guo-Tong Xu 10.1371/journal.pone.0155860.g002 https://plos.figshare.com/articles/figure/Ofd1_ciliary_associated_genes_and_Wnt_signaling_pathway_genes_expression_in_RCS_rat_retina_/3390895 <p>RCS rats were sacrificed between 1 and 6 postnatal weeks. One-week-old (1w) RCS rats were used as the control. (A) <i>Ofd1</i> mRNA expression level increased between the 1st and 4th weeks, and decreased after the 4th week, as determined by qRT-PCR. N = 4. (B) Expression level of Ofd1, Lca5 and Follistatin in RCS rat retina determined using Western blotting analysis. (C) Quantified results of Ofd1, Lca5 and Follistatin protein levels in RCS rats by QuantityOne software. Ofd1 showed the same expression pattern at the mRNA level. Lca5 and Follistatin expression presented the same pattern as Ofd1. (D) Expression level as determined by qRT-PCR of ciliary associated genes <i>Lca5</i>, <i>Fam161a</i>, <i>Rpgrip1</i>, <i>Cep290</i> and Wnt signaling pathway genes: <i>Axin2</i> and <i>Follistatin</i>. N = 4. *P<0.05, **P<0.01. For quantification results, three replicates were performed but only one band for three representative samples per group was shown.</p> 2016-05-19 10:01:54 RP ofd 1 neuroprotection Reactive oxygen species Apoptosis genes expression R 28 cells rat retina photoreceptors MNU ROS 1 st Ofd 1 function retina degeneration models Ofd 1 localization photoreceptor degeneration models rat models photoreceptor cilium length Ofd 1 overexpression rat retina Photoreceptor Degeneration Models Ofd 1 RCS OFD ciliary proteins expression levels pathway proteins expression ICC degeneration rat models Ofd 1 Exhibits Neuroprotective Function Ofd 1 expression FACS