Phylogenetic analysis and genome organization of <i>tatABC</i> genes. Pablo A. Nuñez Marcelo Soria Marisa D. Farber 10.1371/journal.pone.0033605.g001 https://plos.figshare.com/articles/figure/_Phylogenetic_analysis_and_genome_organization_of_tatABC_genes_/337529 <p>(A) Left side panel: Phylogenetic tree construction using neighbor-joining method (NJ-JTT, 1000 bootstrap) with MEGA.4 of 53 bacterial species based on the concatenated alignment of 11 conserved proteins (RNA Pol ß and ß′, alanyl-tRNA synthetase, phenylalanyl-tRNA synthetase, arginyl-tRNA synthetase, EF-Tu, EF-G, RecA, GyrA, Gyrß, Hsp70). Right side panel: Genome organization of <i>tatABC</i> genes. (B) <i>tatA, tatB</i> and <i>tatC</i> genes in <i>Anaplasma marginale str. St. Maries</i>, and <i>Brucella melitensis biovar abortus 2308</i> (Chr I) were displayed and visualized with the Circular Genome Viewer (CGView). Orthologs genes for <i>tatA</i>, <i>tatB</i> and <i>tatC</i> were found in almost all the genomes studied, with the exception of species analyzed from the genera <i>Rickettsia, Neorickettsia, Orientia</i> and <i>Wolbachia</i> that lacked the <i>tatB</i> gene. Three different main organizations according to operon structure preservation were found; one with the commonly described operon organization, another one with a partially dispersed organization (<i>tatA</i> locus maps in a different location from that of the <i>tatBC</i> operon in the same strand), and a completely scattered distribution for <i>tat</i> genes in well-separated location of the circular genomes in several genera of the order Rickettsiales. Organisms lacking a <i>tatB</i> homolog, with the exception of <i>Neorickettsia</i> species, encoded <i>tatA</i> and <i>tatC</i> in different genome strands. The bracket indicates the organisms that share a common <i>tat</i> genes organization.</p> 2012-03-15 02:05:29 genome