miR-34a induces cell cycle arrest and inhibits tumorigenesis in proneural GBM cells. Joachim Silber Anders Jacobsen Tatsuya Ozawa Girish Harinath Alicia Pedraza Chris Sander Eric C. Holland Jason T. Huse 10.1371/journal.pone.0033844.g003 https://plos.figshare.com/articles/figure/_miR_34a_induces_cell_cycle_arrest_and_inhibits_tumorigenesis_in_proneural_GBM_cells_/333243 <p><b>A</b>) 48 hours post-transfection, proneural TS543 were pulsed with BrdU and assayed for cell cycle distribution and <b>B</b>) apoptosis by Annexin V flow cytometry. Staurosporine was added as positive control. <b>C</b>) <i>Ex vivo</i>-transfected TS453 cells stably expressing a luciferase reporter plasmid were xenografted into the brains of ICR scid mice and tumor formation and growth was followed over 11 days. Error bars represent standard deviation within a single experimental set containing multiple replicates. Similar results were obtained in at least 3 independent experiments. *, P<0.05. **, P<0.005.</p> 2012-03-27 00:54:03 induces inhibits tumorigenesis proneural gbm