miR-34a induces cell cycle arrest and inhibits tumorigenesis in proneural GBM cells.
Joachim Silber
Anders Jacobsen
Tatsuya Ozawa
Girish Harinath
Alicia Pedraza
Chris Sander
Eric C. Holland
Jason T. Huse
10.1371/journal.pone.0033844.g003
https://plos.figshare.com/articles/figure/_miR_34a_induces_cell_cycle_arrest_and_inhibits_tumorigenesis_in_proneural_GBM_cells_/333243
<p><b>A</b>) 48 hours post-transfection, proneural TS543 were pulsed with BrdU and assayed for cell cycle distribution and <b>B</b>) apoptosis by Annexin V flow cytometry. Staurosporine was added as positive control. <b>C</b>) <i>Ex vivo</i>-transfected TS453 cells stably expressing a luciferase reporter plasmid were xenografted into the brains of ICR scid mice and tumor formation and growth was followed over 11 days. Error bars represent standard deviation within a single experimental set containing multiple replicates. Similar results were obtained in at least 3 independent experiments. *, P<0.05. **, P<0.005.</p>
2012-03-27 00:54:03
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