10.1371/journal.pone.0033844.g003 Joachim Silber Joachim Silber Anders Jacobsen Anders Jacobsen Tatsuya Ozawa Tatsuya Ozawa Girish Harinath Girish Harinath Alicia Pedraza Alicia Pedraza Chris Sander Chris Sander Eric C. Holland Eric C. Holland Jason T. Huse Jason T. Huse miR-34a induces cell cycle arrest and inhibits tumorigenesis in proneural GBM cells. Public Library of Science 2012 induces inhibits tumorigenesis proneural gbm 2012-03-27 00:54:03 Figure https://plos.figshare.com/articles/figure/_miR_34a_induces_cell_cycle_arrest_and_inhibits_tumorigenesis_in_proneural_GBM_cells_/333243 <p><b>A</b>) 48 hours post-transfection, proneural TS543 were pulsed with BrdU and assayed for cell cycle distribution and <b>B</b>) apoptosis by Annexin V flow cytometry. Staurosporine was added as positive control. <b>C</b>) <i>Ex vivo</i>-transfected TS453 cells stably expressing a luciferase reporter plasmid were xenografted into the brains of ICR scid mice and tumor formation and growth was followed over 11 days. Error bars represent standard deviation within a single experimental set containing multiple replicates. Similar results were obtained in at least 3 independent experiments. *, P<0.05. **, P<0.005.</p>