10.1371/journal.pone.0034389.g006 Shobi Veleri Shobi Veleri Kevin Bishop Kevin Bishop Damian E. Dalle Nogare Damian E. Dalle Nogare Milton A. English Milton A. English Trevor J. Foskett Trevor J. Foskett Ajay Chitnis Ajay Chitnis Raman Sood Raman Sood Paul Liu Paul Liu Anand Swaroop Anand Swaroop Knockdown of <i>Bbs9</i> affects ciliogenesis in IMCD3 cells. Public Library of Science 2012 affects ciliogenesis imcd3 2012-03-29 00:54:00 Figure https://plos.figshare.com/articles/figure/_Knockdown_of_Bbs9_affects_ciliogenesis_in_IMCD3_cells_/333240 <p>(<b>A</b>) <i>Bbs8</i> and <i>Bbs9</i> shRNA transfection in IMCD3 cells. The top row shows eGFP control transfection, whereas middle and bottom rows represent eGFP co-transfected with shRNA against <i>Bbs9</i> or <i>Bbs8</i>, respectively. The nuclei are visualized with DAPI (blue). Transfection is visualized with eGFP (green). Cilia are visualized with both anti-alpha-tubulin and gamma-tubulin (red). shRNA transfected cells (green) have no cilia (red) - highlighted with yellow circle (broken). In the top control panel, eGFP alone-transfected cell shows a cilium (highlighted with yellow arrows). Images are taken at 60× magnification. (<b>B</b>) The quantification of cilia length after <i>Bbs8</i> and <i>Bbs9</i> shRNA transfection in IMCD3 cells (obtained from <b>A</b>). The X and Y axes respectively show transfection category and length (micrometer) of cilia in eGFP transfected cells per seven fields. Data are presented as mean ± SEM, and statistical significance is indicated with p values.</p>