10.1371/journal.pone.0034389.g006
Shobi Veleri
Shobi
Veleri
Kevin Bishop
Kevin
Bishop
Damian E. Dalle Nogare
Damian
E. Dalle Nogare
Milton A. English
Milton
A. English
Trevor J. Foskett
Trevor
J. Foskett
Ajay Chitnis
Ajay
Chitnis
Raman Sood
Raman
Sood
Paul Liu
Paul
Liu
Anand Swaroop
Anand
Swaroop
Knockdown of <i>Bbs9</i> affects ciliogenesis in IMCD3 cells.
Public Library of Science
2012
affects
ciliogenesis
imcd3
2012-03-29 00:54:00
Figure
https://plos.figshare.com/articles/figure/_Knockdown_of_Bbs9_affects_ciliogenesis_in_IMCD3_cells_/333240
<p>(<b>A</b>) <i>Bbs8</i> and <i>Bbs9</i> shRNA transfection in IMCD3 cells. The top row shows eGFP control transfection, whereas middle and bottom rows represent eGFP co-transfected with shRNA against <i>Bbs9</i> or <i>Bbs8</i>, respectively. The nuclei are visualized with DAPI (blue). Transfection is visualized with eGFP (green). Cilia are visualized with both anti-alpha-tubulin and gamma-tubulin (red). shRNA transfected cells (green) have no cilia (red) - highlighted with yellow circle (broken). In the top control panel, eGFP alone-transfected cell shows a cilium (highlighted with yellow arrows). Images are taken at 60× magnification. (<b>B</b>) The quantification of cilia length after <i>Bbs8</i> and <i>Bbs9</i> shRNA transfection in IMCD3 cells (obtained from <b>A</b>). The X and Y axes respectively show transfection category and length (micrometer) of cilia in eGFP transfected cells per seven fields. Data are presented as mean ± SEM, and statistical significance is indicated with p values.</p>