LNA-based antagomiR-22 increased parathymosin 3′UTR reporter activity in a hepatoma cell line Q7 by knockdown endogenous miR-22.
Hung-Lin Chen
Jyun-Yuan Huang
Chun-Ming Chen
Tien-Hua Chu
Chiaho Shih
10.1371/journal.pone.0034116.g008
https://plos.figshare.com/articles/figure/_LNA_based_antagomiR_22_increased_parathymosin_3_8242_UTR_reporter_activity_in_a_hepatoma_cell_line_Q7_by_knockdown_endogenous_miR_22_/327483
<p>(A) The reporter assay was conducted by co-transfection of DsRED-parathymosin 3′UTR with LNA-antagomiR-22 in Q7 cells using Lipofectamine 2000. Reporter activity was significantly increased when 100 pmol of LNA-anti-miR-22 was used. (B) Different concentrations of LNA-antagomiR-22 were used to knockdown the endogenous miR-22 expression level, which was measured by stem-loop real-time PCR. (***, p<0.001) (C) Parathymosin mRNA was increased significantly by stem-loop real-time PCR, when transdifferentiated AR42J-B13 cells were treated with LNA-anti-miR-22. (*, <i>p</i><0.5).</p>
2012-04-06 02:04:43
antagomir-22
parathymosin
hepatoma
q7
knockdown
endogenous