10.1021/la050875x.s001
Songqin Liu
Songqin
Liu
Aicheng Chen
Aicheng
Chen
Coadsorption of Horseradish Peroxidase with Thionine on
TiO<sub>2</sub> Nanotubes for Biosensing
American Chemical Society
2005
spectroscopic measurements show
enzyme immobilization
novel H 2 O 2 sensor
thionine
horseradish peroxidase
anodic oxidation
TiO 2 nanotubes offer
TiO 2 Nanotubes
HRP
nanostructured materials
biosensor design
Horseradish Peroxidase
TiO 2 nanotube arrays
surface areas
0.0 V
TiO 2 nanotubes
H 2 O 2
biocatalytic oxidation
titanium substrate
2005-08-30 00:00:00
Figure
https://acs.figshare.com/articles/figure/Coadsorption_of_Horseradish_Peroxidase_with_Thionine_on_TiO_sub_2_sub_Nanotubes_for_Biosensing/3270901
In this study, we investigate the coadsorption of protein with thionine on TiO<sub>2</sub> nanotubes for biosensor
design. The TiO<sub>2</sub> nanotube arrays fabricated by anodic oxidation of titanium substrate possess large
surface areas and good uniformity and conformability and are ready for enzyme immobilization.
Electrochemical and spectroscopic measurements show that the TiO<sub>2</sub> nanotube arrays provide excellent
matrixes for the coadsorption of horseradish peroxidase (HRP) and thionine and that the adsorbed HRP
on these TiO<sub>2</sub> nanotube arrays effectively retains its bioactivity. The immobilized thionine can be
electrochemically reduced but cannot be reoxidized in the electrode potential range between −0.7 and 0.0
V. The addition of H<sub>2</sub>O<sub>2</sub> leads to the biocatalytic oxidation of the reduced thionine in the presence of HRP,
resulting in developing a novel H<sub>2</sub>O<sub>2</sub> sensor with good stability and reproducibility. The fabricated TiO<sub>2</sub>
nanotubes offer a stage for further study of immobilization and electrochemistry of proteins. The proposed
method opens a way to develop biosensors using nanostructured materials with low electrical conductivity.