10.6084/m9.figshare.3259981
Shraddha Sharma
Shraddha
Sharma
Santosh K. Patnaik
Santosh
K. Patnaik
Zeynep Kemer
Zeynep
Kemer
Bora E. Baysal
Bora
E. Baysal
Transient overexpression of exogenous APOBEC3A causes C-to-U RNA editing of thousands of genes
Taylor & Francis Group
2016
Cytidine deaminase
disease genes
epitranscriptomics
RNA editing
RNA seq
2016-05-05 18:13:37
Dataset
https://tandf.figshare.com/articles/dataset/Transient_overexpression_of_exogenous_APOBEC3A_causes_C_to_U_RNA_editing_of_thousands_of_genes/3259981
<p>APOBEC3A cytidine deaminase induces site-specific C-to-U RNA editing of hundreds of genes in monocytes exposed to hypoxia and/or interferons and in pro-inflammatory macrophages. To examine the impact of APOBEC3A overexpression, we transiently expressed APOBEC3A in HEK293T cell line and performed RNA sequencing. APOBEC3A overexpression induces C-to-U editing at more than 4,200 sites in transcripts of 3,078 genes resulting in protein recoding of 1,110 genes. We validate recoding RNA editing of genes associated with breast cancer, hematologic neoplasms, amyotrophic lateral sclerosis, Alzheimer disease and primary pulmonary hypertension. These results highlight the fundamental impact of APOBEC3A overexpression on human transcriptome by widespread RNA editing.</p>