TY - DATA T1 - A Reagentless Signal-On Architecture for Electronic, Aptamer-Based Sensors via Target-Induced Strand Displacement PY - 2005/12/28 AU - Yi Xiao AU - Brian D. Piorek AU - Kevin W. Plaxco AU - Alan J. Heeger UR - https://acs.figshare.com/articles/journal_contribution/A_Reagentless_Signal_On_Architecture_for_Electronic_Aptamer_Based_Sensors_via_Target_Induced_Strand_Displacement/3249796 DO - 10.1021/ja056555h.s001 L4 - https://ndownloader.figshare.com/files/5084617 KW - Thrombin KW - binding KW - Reagentles KW - alternative KW - methylene KW - MB tag KW - Faradaic KW - gold electrode surface KW - Sensor KW - Strand KW - DNA KW - thrombin concentrations KW - conformation KW - collide KW - nM KW - Displacement KW - Electronic KW - aptamer KW - oligonucleotide N2 - Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as ∼3 nM. ER -