TY - DATA T1 - Different layers of leukocyte infiltration in nodular lesions six weeks after infection. PY - 2016/04/29 AU - Miriam Bolz AU - Nicolas Ruggli AU - Nicole Borel AU - Gerd Pluschke AU - Marie-Thérèse Ruf UR - https://plos.figshare.com/articles/figure/Local_Cellular_Immune_Responses_and_Pathogenesis_of_Buruli_Ulcer_Lesions_in_the_Experimental_i_Mycobacterium_Ulcerans_i_Pig_Infection_Model/3212029 DO - 10.1371/journal.pntd.0004678.s001 L4 - https://ndownloader.figshare.com/files/5042416 KW - pig infection model KW - porcine skin KW - Buruli Ulcer Lesions KW - BU KW - ulcerans bacteria extracellular acid KW - Local Cellular Immune Responses KW - Experimental Mycobacterium Ulcerans Pig Infection Model Background Buruli ulcer N2 - Histological section of a nodular lesion six weeks after infection with 1.3 x 106 CFU of M. ulcerans. Ziehl-Neelsen/Methyleneblue (ZN) staining reveals a strong cellular infiltration around a central necrotic core containing AFB stained in pink (layer 1). The necrotic core (layer 1) is surrounded by three more layers (layers 2–4) of leucocytes differing in their cellular appearance. Optical definition of the layers was done as previously described [16]. (TIF) ER -