%0 Journal Article %A Wang, Jianxin %A Liu, Na %A Cheng, Xiaobo %A Chen, Ligong %D 2016 %T Efficient continuous kinetic resolution of racemic 2-aminobutanol over immobilized penicillin G acylase %U https://tandf.figshare.com/articles/journal_contribution/An_Efficient_Continuous_Kinetic_Resolution_of_Racemic_2_aminobutanol_over_Immobilized_Penicillin_G_Acylase/3205252 %R 10.6084/m9.figshare.3205252 %2 https://ndownloader.figshare.com/files/5030452 %K Acylase %K 2-aminobutanol %K continuous %K hydrolysis %K kinetic resolution %K penicillin G %X

In this paper, an efficient method was established for continuous kinetic resolution of racemic 2-aminobutanol by selective hydrolysis of N-phenylacetyl (±)-2-aminobutanol over immobilized penicillin G acylase (PGA) in a fixed-bed reactor. Several N-acylated derivatives of 2-aminobutanol were screened in batch experiments, and it was found that the hydrolysis of N-phenylacetyl (±)-2-aminobutanol proceeded smoothly in the presence of immobilized penicillin G acylase with satisfied enantioselectivity. Thus, the reaction parameters were optimized in a fixed-bed reactor. Under the optimized conditions, 39.3% conversion of N-phenylacetyl (±)-2-aminobutanol and 98.2% ee value of S-2-aminobutanol were obtained. This fixed-bed system was operated continuously for 40 h without significant decrease of enzyme activity. It has been demonstrated to be more efficient compared to the batch experiments.

%I Taylor & Francis