10.1371/journal.pone.0153956.g001 Tarlan Mamedov Tarlan Mamedov Jessica A. Chichester Jessica A. Chichester R. Mark Jones R. Mark Jones Ananya Ghosh Ananya Ghosh Megan V. Coffin Megan V. Coffin Kristina Herschbach Kristina Herschbach Alexey I. Prokhnevsky Alexey I. Prokhnevsky Stephen J. Streatfield Stephen J. Streatfield Vidadi Yusibov Vidadi Yusibov SDS-PAGE analysis of purified pp-PA83 variants. Public Library of Science 2016 stability candidate Flavobacterium meningosepticum Bacillus anthracis deglycosylated PNGase F version protein production platform anthrax vaccine expression vivo deglycosylation approach Nicotiana benthamiana glycosylated rPA site 2016-04-21 05:56:40 Figure https://plos.figshare.com/articles/figure/SDS_PAGE_analysis_of_purified_pp_PA83_variants_/3194608 <p>Lanes were loaded with 1.75 μg per lane. M: molecular weight markers, A: pp-dPA83 (<i>in vivo</i> deglycosylated), B: glycosylated pp-PA83, C: <i>in vitro</i> deglycosylated pp-PA83, D: pp-PA83M-Q mutant, E: pp-PA83M-D mutant. The arrowhead in lane C indicates migration of PNGase F.</p>