10.1371/journal.pone.0153956.g001
Tarlan Mamedov
Tarlan
Mamedov
Jessica A. Chichester
Jessica
A. Chichester
R. Mark Jones
R.
Mark Jones
Ananya Ghosh
Ananya
Ghosh
Megan V. Coffin
Megan V.
Coffin
Kristina Herschbach
Kristina
Herschbach
Alexey I. Prokhnevsky
Alexey I.
Prokhnevsky
Stephen J. Streatfield
Stephen
J. Streatfield
Vidadi Yusibov
Vidadi
Yusibov
SDS-PAGE analysis of purified pp-PA83 variants.
Public Library of Science
2016
stability
candidate
Flavobacterium meningosepticum Bacillus anthracis
deglycosylated
PNGase F
version
protein production platform
anthrax vaccine
expression
vivo deglycosylation approach
Nicotiana benthamiana
glycosylated
rPA
site
2016-04-21 05:56:40
Figure
https://plos.figshare.com/articles/figure/SDS_PAGE_analysis_of_purified_pp_PA83_variants_/3194608
<p>Lanes were loaded with 1.75 μg per lane. M: molecular weight markers, A: pp-dPA83 (<i>in vivo</i> deglycosylated), B: glycosylated pp-PA83, C: <i>in vitro</i> deglycosylated pp-PA83, D: pp-PA83M-Q mutant, E: pp-PA83M-D mutant. The arrowhead in lane C indicates migration of PNGase F.</p>