Pu-erh Tea Extract Attenuates Nicotine-Induced Foam
Cell Formation in Primary Cultured Monocytes: An in Vitro Mechanistic
Study
Shih-Hsin Tu
Ming-Yao Chen
Li-Ching Chen
Yi-Ting Mao
Chi-Hou Ho
Wen-Jui Lee
Yen-Kuang Lin
Min-Hsiung Pan
Chih-Yu Lo
Chi-Long Chen
Yun Yen
Jacqueline Whang-Peng
Chi-Tang Ho
Chih-Hsiung Wu
Yuan-Soon Ho
10.1021/acs.jafc.6b00624.s001
https://acs.figshare.com/articles/journal_contribution/Pu_erh_Tea_Extract_Attenuates_Nicotine_Induced_Foam_Cell_Formation_in_Primary_Cultured_Monocytes_An_in_Vitro_Mechanistic_Study/3181264
In
this study, the mechanisms by which pu-erh tea extract (PETE)
attenuates nicotine-induced foam cell formation were investigated.
Monocytes were purified from healthy individuals using commercial
antibodies coated with magnetic beads. We found that the nicotine-induced
(1–10 μM) expression of oxidized low-density lipoprotein
receptors (ox-LDLRs) and α9-nAchRs in monocytes was significantly
attenuated by 24 h of PETE (10 μg/mL; ∗, <i>p</i> < 0.05) cotreatment. Nicotine (1 μM for 24 h) significantly
induced the expression of the surface adhesion molecule ICAM-1 and
the monocyte integrin adhesion molecule (CD11b) by human umbilical
vein endothelial cells (HUVECs) and triggered monocytes to differentiate
into macrophages via interactions with the endothelium. After treatment
with nicotine (0.1–10 μM for 24 h), the HUVECs released
chemotactic factors (IL-8) to attract monocytes into the tunica intima
of the artery, and the monocytes then transformed into foam cells.
We demonstrated that PETE treatment (>1 μg/mL for 24 h; ∗, <i>p</i> < 0.05) significantly attenuates nicotine-induced (1
μM) monocyte migration toward HUVECs and foam cell formation.
This study suggests that tea components effectively attenuate the
initial step (foam cell formation) of nicotine-induced atherosclerosis
in circulating monocytes.
2016-03-21 00:00:00
HUVEC
PETE
Vitro Mechanistic Study
Primary Cultured Monocytes
24 h
monocyte
foam cell formation
attenuate