%0 Journal Article %A Schmidt, J. %A Zyba, V. %A Jung, K. %A Rinke, S. %A Haak, R. %A F. Mausberg, R. %A Ziebolz, D. %D 2016 %T Cytotoxic effects of octenidine mouth rinse on human fibroblasts and epithelial cells – an in vitro study %U https://tandf.figshare.com/articles/journal_contribution/Cytotoxic_effects_of_octenidine_mouth_rinse_on_human_fibroblasts_and_epithelial_cells_an_i_in_vitro_i_study/3168280 %R 10.6084/m9.figshare.3168280 %2 https://ndownloader.figshare.com/files/4932685 %K LIS %K epithelial cells %K CHX %K octenidine mouth rinse %K OCT %K BET %K MR %K fibroblast %K 15  min %K MER %X

Objectives: This study compared the cytotoxicity of a new octenidine mouth rinse (MR) against gingival fibroblasts and epithelial cells with different established MRs. Methods: The following MRs were used: Octenidol (OCT), Chlorhexidine 0.2% (CHX), Listerine (LIS), Meridol (MER), Betaisodona (BET); and control (medium only). Human primary gingiva fibroblasts and human primary nasal epithelial cells were cultivated in cell-specific media (2 × 105 cells/ml) and treated with MR for 1, 5, and 15 min. Each test was performed 12 times. Metabolism activity was measured using a cytotoxicity assay. A cellometer analyzed cell viability, cell number, and cell diameter. The data were analyzed by two-way analysis of variance with subsequent Dunnett’s test and additional t-tests. Results: The cytotoxic effects of all MRs on fibroblasts and epithelial cells compared to the control depended on the contact time (p < 0.001). OCT and BET showed less influence on cell metabolism in fibroblasts than other MRs. OCT also demonstrated comparable but not significant results in epithelial cells (p > 0.005). Cell numbers of both cell types at all contact times revealed that OCT showed a less negative effect (p > 0.005), especially for epithelial cells compared to CHX after 15 min (p < 0.005). OCT and BET showed the best results for viability in fibroblasts (p > 0.005), but MER showed less influence than OCT in epithelial cells (p < 0.005). Conclusions: OCT is a potential alternative to CHX regarding cytotoxicity because of its lower cell-toxic effect against fibroblasts and epithelial cells.

%I Taylor & Francis