Golkar, Nasim Mohammad Tamaddon, Ali Mohammadi Samani, Soliman Effect of lipid composition on incorporation of trastuzumab-PEG-lipid into nanoliposomes by post-insertion method: physicochemical and cellular characterization <p><i>Context</i>: Anti-HER2 immunoliposomes are promising nanotechnology based systems for active targeting of breast tumors, which depends on the amount of incorporated antibody.</p> <p><i>Objective/Aim</i>: In this work, we investigated the possible effect of lipid composition on the incorporation of trastuzumab-PEG-PE micelles into nanoliposomes and on their subsequent specific cellular targeting.</p> <p><i>Materials and methods</i>: Trastuzumab (anti-HER2 monoclonal antibody) was monothiolated and conjugated to maleimide-PEG-PE micelles. Liposomes of different lipid compositions were prepared by the thin layer hydration. Trastuzumab-PEG-PE micelles were incorporated into the liposomes by the post-insertion method. The percentage of lipid mixing was determined based on fluorescence resonance energy transfer. Cellular binding and uptake of rhodamine-labeled immunoliposomes were studied in SKBR-3 (HER2<sup>+++</sup>) and MCF-7 (HER2<sup>+</sup>) cells. Also, antitumor cell activity of the immunoliposomes was compared to free trastuzumab and the liposomes.</p> <p><i>Results</i>: The lipid mixing of trastuzumab-PEG-PE micelles depended on the liposome composition. The immunoliposomes containing DPPC, cholesterol and PEG-PE showed prominent lipid mixing. The lipid mixing was consistent with the cell binding results which showed an efficient and specific binding of the immunoliposomes to SKBR-3 cells. Antitumor cell activity of the immunoliposomes in SKBR-3, unlike MCF-7 cells, depended on the content of trastuzumab.</p> <p><i>Discussion</i>: Cholesterol and PEG-PE in the liposome composition are prerequisites for a successful lipid mixing due to their ability to facilitate fusion. The higher lipid mixing results in higher antibody incorporation and consequently higher targeted cell binding.</p> <p><i>Conclusions</i>: The lipid mixing depends on the liposome composition, which reflects targeted cell binding of the immunoliposomes.</p> lipid composition;antitumor cell activity;cell binding results;micelle;cell binding;SKBR;immunoliposome;HER;liposome composition;Antitumor cell activity;fluorescence resonance energy transfer;DPPC;MCF 2016-04-01
    https://tandf.figshare.com/articles/journal_contribution/Effect_of_lipid_composition_on_incorporation_of_trastuzumab_PEG_lipid_into_nanoliposomes_by_post_insertion_method_physicochemical_and_cellular_characterization/3145621
10.6084/m9.figshare.3145621.v1