%0 Journal Article %A Urrego, Diana %A Movsisyan, Naira %A Ufartes, Roser %A A. Pardo, Luis %D 2016 %T Periodic expression of Kv10.1 driven by pRb/E2F1 contributes to G2/M progression of cancer and non-transformed cells %U https://tandf.figshare.com/articles/journal_contribution/Periodic_expression_of_Kv10_1_driven_by_pRb_E2F1_contributes_to_G2_M_progression_of_cancer_and_non_transformed_cells/3142294 %R 10.6084/m9.figshare.3142294.v1 %2 https://ndownloader.figshare.com/files/4885804 %K cell cycle %K potassium channel %K Kv10.1 %K E2F1 %K pRb %X

Progression of cell cycle is associated with changes in K+ channel expression and activity. In this study, we report that Kv10.1, a K+ channel that increases cell proliferation and tumor growth, is regulated at the transcriptional level by the pRb/E2F1 pathway. De-repression of E2F1 by HPV-E7 oncoprotein leads to increased expression of Kv10.1. In proliferating cells, E2F1 transcription factor binds directly to the Kv10.1 promoter during (or close to) G2/M, resulting in transient expression of the channel. Importantly, this happens not only in cancer cells but also in non-transformed cells. Lack of Kv10.1 in both cancer and non-transformed cells resulted in prolonged G2/M phase, as indicated by phosphorylation of Cdk1 (Y15) and sustained pRb hyperphosphorylation. Our results strongly suggest that Kv10.1 expression is coupled to cell cycle progression and facilitates G2/M progression in both healthy and tumor cells.

%I Taylor & Francis