10.1371/journal.pone.0036510.g008 Balraj Singh Balraj Singh Karen Tai Karen Tai Simran Madan Simran Madan Milan R. Raythatha Milan R. Raythatha Amanda M. Cady Amanda M. Cady Megan Braunlin Megan Braunlin LaTashia R. Irving LaTashia R. Irving Ankur Bajaj Ankur Bajaj Anthony Lucci Anthony Lucci Glutamine-independent phenotype is adaptable but stable. Public Library of Science 2012 phenotype adaptable 2012-05-03 01:09:52 Figure https://plos.figshare.com/articles/figure/_Glutamine_independent_phenotype_is_adaptable_but_stable_/314192 <p><b>Top panel:</b> Induction of GLS upon growth in glutamine-containing medium. SUM149-Gln-ind cells growing in Gln-free medium were shifted to Gln-containing medium, and GLS was analyzed by western blotting at different time points after the medium change. <b>Bottom panel:</b> Morphologies of cells growing under different conditions are shown. (A) Gln-ind cells growing without Gln. (B) Gln-ind cells growing in the presence of Gln (they exhibited faster growth and altered morphology upon growth with Gln). (C) Gln-ind cells were maintained in Gln-containing medium for 8 passages and then switched to Gln-free medium for 7 days.</p>