10.1371/journal.pone.0036683.g006
Laura E. Sperling
Laura
E. Sperling
Janine Klaczinski
Janine
Klaczinski
Corina Schütz
Corina
Schütz
Lydia Rudolph
Lydia
Rudolph
Paul G. Layer
Paul
G. Layer
PRiMA overexpression in a stably AChE-transfected R28 cell
Public Library of Science
2013
overexpression
stably
ache-transfected
r28
2013-02-20 05:38:42
Figure
https://plos.figshare.com/articles/figure/_PRiMA_overexpression_in_a_stably_AChE_transfected_R28_cell_/314191
<p>line <b>localizes AChE to cell membrane and changes cell morphology</b>. Note strong AChE activity on membrane of PRiMA transfected cells, with multiple emanating short side processes (C, D), as compared with more diffuse, but still localized AChE expression in control cells (A, B). (A–D) Karnovsky-Roots staining; all cells were grown on laminin-1. Scale bar 50 µm. Fig. 6 (lower). Expression of PRiMA is strongly increased in cells overexpressing AChE, and more so in AChE plus PRiMA co-transfected cells, as detected by RT-PCR. Total RNA of control, of AChE transfected, and of AChE plus PRiMA transfected cells was used for the analyses. Note: GAPDH primers were used as internal control.</p>