Endoglin expression during <i>in vitro</i> differentiation of murine Mo towards MΦ.
Luisa Ojeda-Fernández
Lucía Recio-Poveda
Mikel Aristorena
Pedro Lastres
Francisco J. Blanco
Francisco Sanz-Rodríguez
Eunate Gallardo-Vara
Mateo de las Casas-Engel
Ángel Corbí
Helen M. Arthur
Carmelo Bernabeu
Luisa M. Botella
10.1371/journal.pgen.1005935.g001
https://plos.figshare.com/articles/figure/Endoglin_expression_during_i_in_vitro_i_differentiation_of_murine_Mo_towards_M_/3139378
<p><b>(A)</b> Induction of endoglin expression during <i>in vitro</i> cultured blood monocytes. Blood samples from a total of 10 mice for each condition were pooled. Adherent cells were trypsinized at selected times and flow cytometry analysis was used to determine endoglin expression on cultured Mo, defined as CD11b<sup>pos</sup> Ly6G<sup>neg</sup> cells. <b>(B)</b> Representative flow cytometry graphs of endoglin expression on cultured Mo are shown by profiles with black line. Grey profiles represent the negative control. Percentage of endoglin positive cells is shown in the upper right corner of each histogram <b>(C)</b> Endoglin mRNA expression levels in BMDMs differentiated to M1 or M2 macrophages. Expression levels of <i>Eng</i> mRNA in BMDMs treated with GM-CSF (M1) or M-CSF (M2) from 3 different C57BL/6 donors. Relative <i>Eng</i> mRNA levels are normalized to <i>18S</i> as endogenous controls. Mean and SD of each triplicate are shown; ** <i>P</i><0.01 Ms = mouse. <b>(D)</b> Endoglin expression levels on BMDMs differentiated to M1 or M2 phenotype from 3 different C57BL/6 mice, with each sample analyzed in duplicate. On the right are representative flow cytometry results showing endoglin expression in BMDMs differentiated to M1 or M2 phenotype. Percentage of positive endoglin cells is shown in the upper right corner of each histogram. Grey profiles represent the negative control.</p>
2016-03-24 06:52:53
endoglin expression
TGF
Impaired Immune Response Endoglin
Macrophages Show
57BL
ENG
vessel wall
Phagocytic activity
mice show
vivo differentiation
endoglin myeloid lineage
endoglin gene
Hereditary Hemorrhagic Telangiectasia
role
HHT 1 patients
blood monocytes
mouse line
macrophage function
ACVRL