10.1371/journal.pgen.1005935.g001 Luisa Ojeda-Fernández Luisa Ojeda-Fernández Lucía Recio-Poveda Lucía Recio-Poveda Mikel Aristorena Mikel Aristorena Pedro Lastres Pedro Lastres Francisco J. Blanco Francisco J. Blanco Francisco Sanz-Rodríguez Francisco Sanz-Rodríguez Eunate Gallardo-Vara Eunate Gallardo-Vara Mateo de las Casas-Engel Mateo de las Casas-Engel Ángel Corbí Ángel Corbí Helen M. Arthur Helen M. Arthur Carmelo Bernabeu Carmelo Bernabeu Luisa M. Botella Luisa M. Botella Endoglin expression during <i>in vitro</i> differentiation of murine Mo towards MΦ. Public Library of Science 2016 endoglin expression TGF Impaired Immune Response Endoglin Macrophages Show 57BL ENG vessel wall Phagocytic activity mice show vivo differentiation endoglin myeloid lineage endoglin gene Hereditary Hemorrhagic Telangiectasia role HHT 1 patients blood monocytes mouse line macrophage function ACVRL 2016-03-24 06:52:53 Figure https://plos.figshare.com/articles/figure/Endoglin_expression_during_i_in_vitro_i_differentiation_of_murine_Mo_towards_M_/3139378 <p><b>(A)</b> Induction of endoglin expression during <i>in vitro</i> cultured blood monocytes. Blood samples from a total of 10 mice for each condition were pooled. Adherent cells were trypsinized at selected times and flow cytometry analysis was used to determine endoglin expression on cultured Mo, defined as CD11b<sup>pos</sup> Ly6G<sup>neg</sup> cells. <b>(B)</b> Representative flow cytometry graphs of endoglin expression on cultured Mo are shown by profiles with black line. Grey profiles represent the negative control. Percentage of endoglin positive cells is shown in the upper right corner of each histogram <b>(C)</b> Endoglin mRNA expression levels in BMDMs differentiated to M1 or M2 macrophages. Expression levels of <i>Eng</i> mRNA in BMDMs treated with GM-CSF (M1) or M-CSF (M2) from 3 different C57BL/6 donors. Relative <i>Eng</i> mRNA levels are normalized to <i>18S</i> as endogenous controls. Mean and SD of each triplicate are shown; ** <i>P</i><0.01 Ms = mouse. <b>(D)</b> Endoglin expression levels on BMDMs differentiated to M1 or M2 phenotype from 3 different C57BL/6 mice, with each sample analyzed in duplicate. On the right are representative flow cytometry results showing endoglin expression in BMDMs differentiated to M1 or M2 phenotype. Percentage of positive endoglin cells is shown in the upper right corner of each histogram. Grey profiles represent the negative control.</p>